Seth R, Motte P, Kehely A, Wimalawansa S, Self C H, Bohuon C, Bellet D, MacIntyre I
Department of Chemical Pathology, Royal Postgraduate Medical School, London, United Kingdom.
Horm Metab Res Suppl. 1989;21:3-5.
We have developed a sensitive, two-site enzyme immunometric assay (EIA) using high affinity monoclonal antibodies for the measurement of immunoreactive human calcitonin (i-hCT) and compared the performance of the assay with that of the conventional radioimmunoassay (RIA). The sensitivity of an overnight EIA (2 pmol/l) was comparable with the long incubation (7 days) RIA. Both, the EIA and the RIA have been applied for the measurement of i-hCT in three groups: normal volunteers (n = 53), post-menopausal women (n = 25) and patients with MTC (n = 20). The results showed that in all three groups, the level of i-hCT was somewhat lower than that measured by RIA due to its greater specificity. The EIA can provide an attractive alternative to the conventional RIA for both routine diagnostic work and physiological studies.
我们已经开发出一种灵敏的双位点酶免疫测定法（EIA），该方法使用高亲和力单克隆抗体来测量免疫反应性人降钙素（i-hCT），并将该测定法的性能与传统放射免疫测定法（RIA）进行了比较。过夜EIA（2 pmol/l）的灵敏度与长时间孵育（7天）的RIA相当。EIA和RIA都已应用于三组人群i-hCT的测量：正常志愿者（n = 53）、绝经后女性（n = 25）和甲状腺髓样癌患者（n = 20）。结果表明，在所有三组中，由于EIA具有更高的特异性，其测量的i-hCT水平略低于RIA测量的水平。对于常规诊断工作和生理学研究而言，EIA是传统RIA颇具吸引力的替代方法。
