Wang Fei, Deng Lianfu, Hu Jingjing, Cheng Yiyun
Shanghai Key Laboratory of Regulatory Biology and School of Life Sciences, East China Normal University , Shanghai 200241, China.
Shanghai Key Laboratory for Bone and Joint Diseases, Shanghai Institute of Traumatology and Orthopaedics, Shanghai Ruijin Hospital, School of Medicine, Shanghai Jiaotong University , Shanghai 200025, China.
Bioconjug Chem. 2016 Mar 16;27(3):638-46. doi: 10.1021/acs.bioconjchem.5b00643. Epub 2016 Feb 5.
Multifunctionality is necessary in the design of efficient gene vectors due to the existence of multiple barriers during gene delivery. Traditional methods in the design of polyfunctional materials for this purpose are associated with sophisticated syntheses and high costs. Here, we proposed a facile coassembly approach to fabricate multicomponent nanoparticles for efficient gene delivery. The resulting particles contain different functional dendrimers and show favorable physicochemical characteristics. All the combinations in the fabrication of multicomponent nanoparticles show a synergistic effect in improving transfection efficacy. The prepared nanoparticles successfully address two or more barriers in the gene delivery process and show minimal toxicity on the transfected cells. The combination of high transfection efficacy and low cytotoxicity suggests that the prepared multicomponent nanoparticles as promising carriers in gene delivery.
由于基因递送过程中存在多种障碍，因此在高效基因载体的设计中多功能性是必要的。为此目的设计多功能材料的传统方法涉及复杂的合成和高成本。在这里，我们提出了一种简便的共组装方法来制备用于高效基因递送的多组分纳米颗粒。所得颗粒包含不同的功能性树枝状大分子，并显示出良好的物理化学特性。多组分纳米颗粒制备中的所有组合在提高转染效率方面都显示出协同效应。所制备的纳米颗粒成功地解决了基因递送过程中的两个或更多个障碍，并且对转染细胞显示出最小的毒性。高转染效率和低细胞毒性的结合表明，所制备的多组分纳米颗粒有望成为基因递送的载体。
