软骨内骨化中X型胶原蛋白的免疫电子显微镜研究。
Immunoelectron microscopic studies of type X collagen in endochondral ossification.
作者信息
Poole A R, Pidoux I
机构信息
Joint Diseases Laboratory, Shriners Hospital for Crippled Children, Montreal, Quebec, Canada.
出版信息
J Cell Biol. 1989 Nov;109(5):2547-54. doi: 10.1083/jcb.109.5.2547.
Abstract
Immunofluorescence and immunoelectron microscopy were used in conjunction with a monoclonal antibody to investigate the localization of type X collagen in the proximal tibial growth plate of 7-d-old chicks. This molecule was detected throughout the hypertrophic zone first appearing when chondrocytes exhibited hypertrophy: it was absent from the proliferative zone. Type X collagen was primarily associated with type II collagen fibrils as demonstrated by immunogold staining. Type X collagen was not concentrated in the focal calcification sites nor was it associated with matrix vesicles. These observations suggest that type X collagen may play a role other than that directly related to the nucleation of calcification.
摘要
免疫荧光和免疫电子显微镜技术结合单克隆抗体，用于研究X型胶原蛋白在7日龄雏鸡胫骨近端生长板中的定位。当软骨细胞出现肥大时，在整个肥大区均可检测到该分子，而增殖区则未检测到。免疫金染色显示，X型胶原蛋白主要与II型胶原纤维相关。X型胶原蛋白并不集中在局灶性钙化部位，也不与基质小泡相关。这些观察结果表明，X型胶原蛋白可能发挥着与钙化成核直接相关作用以外的其他作用。
相似文献
1
Immunoelectron microscopic studies of type X collagen in endochondral ossification.软骨内骨化中X型胶原蛋白的免疫电子显微镜研究。
J Cell Biol. 1989 Nov;109(5):2547-54. doi: 10.1083/jcb.109.5.2547.
2
Association of an extracellular protein (chondrocalcin) with the calcification of cartilage in endochondral bone formation.一种细胞外蛋白（软骨钙素）与软骨内骨形成过程中软骨钙化的关联。
J Cell Biol. 1984 Jan;98(1):54-65. doi: 10.1083/jcb.98.1.54.
3
Primary culture of rat growth plate chondrocytes: an in vitro model of growth plate histotype, matrix vesicle biogenesis and mineralization.大鼠生长板软骨细胞的原代培养：生长板组织类型、基质小泡生物发生及矿化的体外模型
Bone. 2004 Jun;34(6):961-70. doi: 10.1016/j.bone.2004.02.010.
4
Ultrastructural organization of type XI collagen in fetal bovine epiphyseal cartilage.胎牛骨骺软骨中XI型胶原蛋白的超微结构组织
Histochemistry. 1993 Sep;100(3):231-9. doi: 10.1007/BF00269096.
5
Remodelling of collagen types I, II and X and calcification of human fetal cartilage.人胎儿软骨中I型、II型和X型胶原蛋白的重塑及钙化
Bone Miner. 1992 Aug;18(2):107-17. doi: 10.1016/0169-6009(92)90851-4.
6
The association of a newly discovered protein, called chondrocalcin, with cartilage calcification.一种新发现的名为软骨钙素的蛋白质与软骨钙化之间的关联。
Acta Biol Hung. 1984;35(2-4):143-9.
7
Distribution of type X collagen in tibiotarsi of broiler chickens with vitamin D deficiency.
Calcif Tissue Int. 1991 Sep;49(3):188-92. doi: 10.1007/BF02556116.
8
Ultrastructural studies of the epiphyseal plate of chicks fed a vitamin D-deficient and low-calcium diet.对喂食维生素D缺乏和低钙饮食的雏鸡骨骺板的超微结构研究。
J Comp Pathol. 1995 Aug;113(2):101-11. doi: 10.1016/s0021-9975(05)80026-x.
9
Type X collagen in avian tibial dyschondroplasia.
Lab Invest. 1989 Jan;60(1):106-12.
10
Matrix vesicles and focal proteoglycan aggregates are the nucleation sites revealed by the lanthanum incubation method: a correlated study on the hypertrophic zone of the rat epiphyseal cartilage.基质小泡和局灶性蛋白聚糖聚集体是镧孵育法揭示的成核位点：对大鼠骨骺软骨肥大区的相关研究
Calcif Tissue Int. 1996 Apr;58(4):273-82. doi: 10.1007/BF02508648.
引用本文的文献
1
Respiratory chain inactivation links cartilage-mediated growth retardation to mitochondrial diseases.呼吸链失活将软骨介导的生长迟缓与线粒体疾病联系起来。
J Cell Biol. 2019 Jun 3;218(6):1853-1870. doi: 10.1083/jcb.201809056. Epub 2019 May 13.
2
Hypoxia-inducible factor-2α induces expression of type X collagen and matrix metalloproteinases 13 in osteoarthritic meniscal cells.缺氧诱导因子-2α诱导骨关节炎半月板细胞中X型胶原蛋白和基质金属蛋白酶13的表达。
Inflamm Res. 2016 Jun;65(6):439-48. doi: 10.1007/s00011-016-0926-1. Epub 2016 Feb 18.
3
The constitutive expression of type x collagen in mesenchymal stem cells from osteoarthritis patients is reproduced in a rabbit model of osteoarthritis.骨关节炎患者骨髓间充质干细胞中 X 型胶原的组成性表达在骨关节炎兔模型中得到重现。
J Tissue Eng. 2011;2011:587547. doi: 10.4061/2011/587547. Epub 2011 Jul 25.
4
A dominant interference collagen X mutation disrupts hypertrophic chondrocyte pericellular matrix and glycosaminoglycan and proteoglycan distribution in transgenic mice.显性干扰性胶原蛋白X突变破坏转基因小鼠肥大软骨细胞的细胞周基质以及糖胺聚糖和蛋白聚糖的分布。
Am J Pathol. 2001 Dec;159(6):2257-69. doi: 10.1016/S0002-9440(10)63076-3.
5
In situ hybridisation study of type I, II, X collagens and aggrecan mRNas in the developing condylar cartilage of fetal mouse mandible.胎鼠下颌髁突软骨发育过程中I型、II型、X型胶原蛋白和聚集蛋白聚糖mRNA的原位杂交研究
J Anat. 1999 Oct;195 ( Pt 3)(Pt 3):321-9. doi: 10.1046/j.1469-7580.1999.19530321.x.
6
Immunohistochemistry of collagen types II and X, and enzyme-histochemistry of alkaline phosphatase in the developing condylar cartilage of the fetal mouse mandible.胎鼠下颌髁突软骨发育过程中Ⅱ型和X型胶原蛋白的免疫组织化学及碱性磷酸酶的酶组织化学研究
J Anat. 1997 Nov;191 ( Pt 4)(Pt 4):561-70. doi: 10.1046/j.1469-7580.1997.19140561.x.
7
Abnormal compartmentalization of cartilage matrix components in mice lacking collagen X: implications for function.缺乏胶原蛋白X的小鼠软骨基质成分的异常分隔：对功能的影响。
J Cell Biol. 1997 Jan 27;136(2):459-71. doi: 10.1083/jcb.136.2.459.
8
Retarded skeletal development in transgenic mice with a type II collagen mutation.患有II型胶原蛋白突变的转基因小鼠骨骼发育迟缓。
Am J Pathol. 1996 Dec;149(6):2169-82.
9
Localization of collagens and alkaline phosphatase activity during mineralization and ossification of human first rib cartilage.人第一肋软骨矿化和骨化过程中胶原蛋白及碱性磷酸酶活性的定位
Histochem Cell Biol. 1996 Mar;105(3):213-9. doi: 10.1007/BF01462294.
10
Vitamin E stimulates trabecular bone formation and alters epiphyseal cartilage morphometry.维生素E刺激小梁骨形成并改变骨骺软骨形态学。
Calcif Tissue Int. 1995 Oct;57(4):293-300. doi: 10.1007/BF00298885.
本文引用的文献
1
The development and phosphatase activity in vivo and in vitro of the mandibular skeletal tissue of the embryonic fowl.胚胎期雏鸡下颌骨骼组织在体内和体外的发育及磷酸酶活性
Biochem J. 1930;24(6):1905-21. doi: 10.1042/bj0241905.
2
The growth, development and phosphatase activity of embryonic avian femora and limb-buds cultivated in vitro.体外培养的鸡胚股骨和肢芽的生长、发育及磷酸酶活性
Biochem J. 1929;23(4):767-784.5. doi: 10.1042/bj0230767.
3
The Possible Significance of Hexosephosphoric Esters in Ossification.己糖磷酸酯在骨化中的可能意义。
Biochem J. 1923;17(2):286-93. doi: 10.1042/bj0170286.
4
Immunity to homologous cartilage proteoglycans in rabbits with chronic inflammatory arthritis.患有慢性炎症性关节炎的兔子对同源软骨蛋白聚糖的免疫反应
Coll Relat Res. 1981 Sep;1(5):453-73. doi: 10.1016/s0174-173x(81)80029-5.
5
6
A unique low molecular weight collagen secreted by cultured chick embryo chondrocytes.一种由培养的鸡胚软骨细胞分泌的独特的低分子量胶原蛋白。
J Biol Chem. 1982 Oct 25;257(20):12444-50.
7
Electron optical and analytical observations of rat growth plate cartilage prepared by ultracryomicrotomy: the failure to detect a mineral phase in matrix vesicles and the identification of heterodispersed particles as the initial solid phase of calcium phosphate deposited in the extracellular matrix.超低温切片制备的大鼠生长板软骨的电子光学和分析观察：未能在基质小泡中检测到矿物相，并将异质分散颗粒鉴定为沉积在细胞外基质中的磷酸钙的初始固相。
J Ultrastruct Res. 1982 Mar;78(3):227-68. doi: 10.1016/s0022-5320(82)80001-4.
8
Effects of matrix macromolecules on chondrocyte gene expression: synthesis of a low molecular weight collagen species by cells cultured within collagen gels.基质大分子对软骨细胞基因表达的影响：在胶原凝胶中培养的细胞合成低分子量胶原种类
J Cell Biol. 1982 Jun;93(3):767-74. doi: 10.1083/jcb.93.3.767.
9
Molecular structure of short-chain (SC) cartilage collagen by electron microscopy.通过电子显微镜观察短链（SC）软骨胶原蛋白的分子结构。
J Ultrastruct Res. 1984 Feb;86(2):186-91. doi: 10.1016/s0022-5320(84)80057-x.
10
Matrix vesicle calcification in rat epiphyseal growth plate cartilage prepared anhydrously for electron microscopy.
Metab Bone Dis Relat Res. 1983;5(3):131-7. doi: 10.1016/0221-8747(83)90014-0.
Zotero 插件