Ferreira L C L, Cooke R F, Marques R S, Fernandes H J, Fernandes C E, Stelato R, Franco G L, Lemos R A A
J Anim Sci. 2016 Jan;94(1):401-5. doi: 10.2527/jas.2015-9537.
This study compared reproductive performance of cows vaccinated against the foot-and-mouth disease (FMD) virus before timed AI or during early pregnancy (Exp. 1), as well as rectal temperature (RT) and plasma concentrations of the acute-phase protein haptoglobin in cattle vaccinated or not against the FMD virus (Exp. 2). Cattle utilized in Exp. 1 and 2 originated from herds with no historical occurrences of FMD and that received vaccination against the FMD virus biannually. In Exp. 1, 604 lactating, multiparous, nonpregnant Nelore cows were randomly assigned on d -31 of the experiment to receive 1) vaccination against the FMD virus on d ≥ 31 (VACPRE; = 291) and 2) vaccination against FMD virus on d 30 (VACGEST; = 313). From d -11 to 0, all cows were assigned to an estrus synchronization + timed AI (d 0) protocol. Pregnancy status to AI was verified on d 30 and 90 via transrectal ultrasonography. A treatment × day interaction was detected ( < 0.01) for pregnancy rates to AI, which were similar ( = 0.17) between VACPRE and VACGEST on d 30 (61.8% vs. 56.2%, respectively; SEM = 2.8) but greater ( < 0.01) for VACPRE on d 90 (59.4% vs. 46.9%, respectively; SEM = 2.8). Pregnancy loss from d 30 to 90 was greater ( < 0.01) in VACGEST compared with VACPRE (16.5% vs. 3.9%, respectively; SEM = 2.2). In Exp. 2, 40 pregnant Nelore females (20 nulliparous and 20 multiparous cows; BCS = 4.73 ± 0.12) were ranked by parity and assigned to receive (VAC; = 20) or not receive (NOVAC; = 20) vaccination against the FMD virus. Blood samples were collected and RT was recorded before (h 0) and 24, 72, 120, and 168 h after treatment administration. Treatment × day interactions were detected ( < 0.01) for RT and plasma haptoglobin. The RT was greater ( < 0.01) in VAC compared with NOVAC at 24 h after treatment administration and was similar ( ≥ 0.31) between treatments at all other sampling hours. Plasma haptoglobin concentration was similar ( = 0.98) between VAC and NOVAC before treatment administration ( = 0.48) and greater ( < 0.01) in VAC at 24, 72, 120, and 168 h after treatment administration. In summary, vaccinating beef cows against the FMD virus resulted in a 4-fold increase in pregnancy loss when the vaccine was administered 30 d after timed AI compared with 31 d before timed AI. These outcomes can be associated with inflammatory and acute-phase reactions elicited by the FMD vaccine, which are known to impair pregnancy maintenance in cattle.
本研究比较了在定时人工授精前或妊娠早期接种口蹄疫（FMD）病毒疫苗的母牛的繁殖性能（实验1），以及接种或未接种FMD病毒疫苗的牛的直肠温度（RT）和急性期蛋白触珠蛋白的血浆浓度（实验2）。实验1和2中使用的牛来自无FMD历史发生记录且每半年接种一次FMD病毒疫苗的牛群。在实验1中，604头泌乳、经产、未怀孕的内洛尔母牛在实验的第-31天被随机分配，以接受1）在第≥31天接种FMD病毒疫苗（VACPRE；n = 291）和2）在第30天接种FMD病毒疫苗（VACGEST；n = 313）。从第-11天到第0天，所有母牛都被分配到一个发情同步+定时人工授精（第0天）方案。在第30天和第90天通过经直肠超声检查确认人工授精后的妊娠状态。检测到人工授精妊娠率存在处理×天数交互作用（P < 0.01），在第30天VACPRE和VACGEST之间的妊娠率相似（P = 0.17）（分别为61.8%和56.2%；标准误 = 2.8），但在第90天VACPRE的妊娠率更高（P < 0.01）（分别为59.4%和46.9%；标准误 = 2.8）。与VACPRE相比，VACGEST从第30天到第90天的妊娠损失更大（P < 0.01）（分别为16.5%和3.9%；标准误 = 2.2）。在实验2中，40头怀孕的内洛尔母牛（20头初产母牛和20头经产母牛；体况评分 = 4.73 ± 0.12）按胎次排序并分配接受（VAC；n = 20）或不接受（NOVAC；n = 20）FMD病毒疫苗接种。在处理给药前（0小时）以及给药后24、72、120和168小时采集血样并记录直肠温度。检测到直肠温度和血浆触珠蛋白存在处理×天数交互作用（P < 0.01）。给药后24小时，VAC组的直肠温度高于NOVAC组（P < 0.01），在所有其他采样时间处理组之间相似（P ≥ 0.31）。处理给药前VAC组和NOVAC组的血浆触珠蛋白浓度相似（P = 0.
Zotero 插件