[帕金森病患者血浆氧化应激生物标志物水平变化及其与认知功能关系的研究]

[Study on changes of plasma levels of oxidative stress biomarkers and its relation with cognition function in patients with parkinson's disease].

作者信息

Zhou Hong, Yuan Yongsheng, Qi Zhiqiang, Tong Qing, Zhang Kezhong

机构信息

Department of Neurology, Jiangsu Shengze Hospital, the Hospital Affiliated to Nanjing Medical University, Suzhou 215228, China.

Department of Neurology, the First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China, Email:

出版信息

Zhonghua Yi Xue Za Zhi. 2015 Nov 3;95(41):3357-60.

PMID:26812976

Abstract

OBJECTIVE

To study the changes of plasma levels of oxidative stress biomarkers in patients with parkinson's disease (PD), and to explore its association with cognition function.

METHODS

Seventy-two PD patients from June 2013 to May 2012 were enrolled. All of them were outpatients or inpatients at the First Affiliated Hospital of Nanjing Medical University. And forty-five age- and gender- matched healthy subjects were used as controls. The information including gender, age, illness duration, years of education and Hoehn & Yahr (H-Y) stage were recorded. Cognition function of all the patients with PD and the controls were measured by using Montreal Cognitive Assessment (MoCA) scale. Plasma levels of catalase (CAT), total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px), total glutathione (T-GSH) and malondialdehyde (MDA) were measured by ELISA . Then we compared and analyzed the results.

RESULTS

Plasma levels of CAT, T-SOD and T-GSH in PD group were significantly lower than the control group [(159 ± 9) kU/L vs (170 ± 5) kU/L, P< 0.01; (97 ± 24) kU/L vs (124 ± 25) kU/L, P<0.01; (17 ± 10) µmol/L vs (60 ± 51) µmol/L, P< 0.01]. Plasma levels of CAT, T-GSH, GSH-Px and MDA were no differences between early PD group (H-Y stage I-II) and middle-late PD group (H-Y stage III or higher) of patients (P>0.05). Plasma levels of T-GSH in PD group with mild cognitive impairment (MCI) were obviously lower than PD group without MCI [(14 ± 7) µmol/L vs (19 ± 11) µmol/L, P< 0.05]. In PD patients, MoCA scores were positively correlated with years of education (β=0.634, P= 0.000) and plasma levels of T-GSH (β= 0.204, P= 0.014), and were negatively correlated with H-Y stage (β=-0.194, P=0.020).

CONCLUSIONS

The damage of plasma antioxidant mechanism may be involved in the pathogenesis of patients with PD. Decrease in plasma levels of T-GSH may be associated with MCI in PD patients . Plasma levels of T-GSH may be a potential early predictive index in PD patients with cognitive dysfunction.

摘要

目的

研究帕金森病（PD）患者血浆氧化应激生物标志物水平的变化，并探讨其与认知功能的关系。

方法

选取2013年6月至2012年5月的72例PD患者，均为南京医科大学第一附属医院的门诊或住院患者。选取45例年龄、性别匹配的健康受试者作为对照。记录性别、年龄、病程、受教育年限和霍恩&雅尔（H-Y）分期等信息。采用蒙特利尔认知评估（MoCA）量表对所有PD患者和对照对照者和对照者的认知功能进行评估。采用酶联免疫吸附测定法（ELISA）检测血浆过氧化氢酶（CAT）、总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）、总谷胱甘肽（T-GSH）和丙二醛（MDA）水平。然后对结果进行比较和分析。

结果

PD组血浆CAT、T-SOD和T-GSH水平显著低于对照组[（159±9）kU/L对（170±5）kU/L，P<0.01；（97±24）kU/L对（124±25）kU/L，P<0.01；（17±10）μmol/L对（60±51）μmol/L，P<0.01]。PD患者早期组（H-Y分期I-II）和中晚期组（H-Y分期III及以上）血浆CAT、T-GSH、GSH-Px和MDA水平比较，差异无统计学意义（P>0.05）。轻度认知障碍（MCI）的PD组血浆T-GSH水平明显低于无MCI的PD组[（14±7）μmol/L对（19±11）μmol/L，P<0.05]。在PD患者中，MoCA评分与受教育年限（β=0.634，P=0.000）和血浆T-GSH水平（β=0.204，P=0.014）呈正相关，与H-Y分期（β=-0.194，P=0.020）呈负相关。