Xiao-Li Wang, Liang Li, Hui Zhang, Xing Wei, Zhi-Yong Tao, Hui Xia, Yuan-Yuan Wang, Xiao-di Yang, Qiang Fang
Department of Microbiology and Parasitology, Bengbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bengbu 233030, China.
Department of Food and Biological engineering, Bengbu College, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 Dec 26;29(1):48-52. doi: 10.16250/j.32.1374.2016196.
To study the effect of exogenous nitric oxide donor sodium nitroprusside (SNP) on antioxidant enzymes activities and lipid peroxidation of mice infected with .
BALB/c mice were infected with separated by the digestion method. Forty-two days post-infection, the peripheral blood and hepatic tissue from the infected or normal mice were collected. Then 4 groups were set：liver homogenate from infected mice + SNP (Group A), liver homogenate from normal mice + SNP (Group B), peripheral blood from infected mice + SNP (Group C), and peripheral blood from normal mice + SNP (Group D). The final concentrations of SNP in each group were set as 0 (blank control), 2, 5, 10 μmol/L and 30 μmol/L, respectively. After reacting with SNP at 37 ℃ for 30 min, the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT) activities, and malondialdehyde (MDA) concentration were examined and compared.
The levels of SOD, CAT, GSH-Px and MDA concentration in the liver and the blood from the mice infected with were significantly higher than those of the normal ones (all < 0.05). When reacted with 10 μmol/L and 30 μmol/L SNP, the SOD, GSH-Px, and CAT activities in Group A and B decreased significantly (all < 0.05), while the liver MDA concentration reacted with 2-30 μmol/L SNP increased obviously (all < 0.05). As reacted with 30 μmol/L SNP, the activities of blood SOD, GSH-Px, and CAT in Group C and D decreased, while the MDA concentration in blood still increased (all < 0.01). When the SNP concentration was in the range of 2-30 μmol/L, there were a negative correlation between the SNP concentrations and SOD, GSH-Px, and CAT activities, as well as a positive correlation with the MDA concentration in the liver and blood from the mice infected with (all < 0.05).
infection could cause oxidative damage to mice, and increase SOD, GSH-Px, and CAT activities. Nitric oxide released from SNP can decrease antioxidase activities, and inhibit the antioxidant capacity of mice infected with .
研究外源性一氧化氮供体硝普钠（SNP）对感染[具体感染物未明确]小鼠抗氧化酶活性及脂质过氧化的影响。
采用消化法分离[具体感染物未明确]感染BALB/c小鼠。感染后42天，采集感染小鼠和正常小鼠的外周血及肝组织。然后设4组：感染小鼠肝匀浆+SNP（A组）、正常小鼠肝匀浆+SNP（B组）、感染小鼠外周血+SNP（C组）、正常小鼠外周血+SNP（D组）。每组SNP最终浓度分别设为0（空白对照）、2、5、10 μmol/L和30 μmol/L。在37℃与SNP反应30分钟后，检测并比较超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）活性及丙二醛（MDA）浓度。
感染[具体感染物未明确]小鼠肝脏和血液中的SOD、CAT、GSH-Px水平及MDA浓度均显著高于正常小鼠（均P<0.05）。与10 μmol/L和30 μmol/L SNP反应时，A组和B组的SOD、GSH-Px及CAT活性显著降低（均P<0.05），而与2 - 30 μmol/L SNP反应时肝脏MDA浓度明显升高（均P<0.05）。与30 μmol/L SNP反应时，C组和D组血液中SOD、GSH-Px及CAT活性降低，而血液中MDA浓度仍升高（均P<0.01）。当SNP浓度在2 - 30 μmol/L范围内时，感染[具体感染物未明确]小鼠肝脏和血液中SNP浓度与SOD、GSH-Px及CAT活性呈负相关，与MDA浓度呈正相关（均P<0.05）。
[具体感染物未明确]感染可导致小鼠氧化损伤，并增加SOD、GSH-Px及CAT活性。SNP释放的一氧化氮可降低抗氧化酶活性，抑制感染[具体感染物未明确]小鼠的抗氧化能力。
