Koury Josely C, Daleprane Julio B, Pitaluga-Filho Mario V, de Oliveira Cyntia F, Gonçalves Mariana C, Passos Magna C F
Nutrition Institute, State University of Rio de Janeiro Brazil, Laboratory of Biochemistry and Nutrition.
J Strength Cond Res. 2016 Feb;30(2):454-60. doi: 10.1519/JSC.0000000000001102.
This study aimed to compare the biochemical markers of muscle and liver injury and total antioxidant capacity in army cadets after a traditional army physical training program and to correlate these effects with aerobic conditioning. Male army cadets (n = 87; age, 20 ± 2 years) were evaluated 12 hours before the start of training (T0), 12 hours after a 30-km march (T1), and 48 hours after military training (T2). Creatine kinase (CK), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) serum levels were measured using an autoanalyzer. Total antioxidant capacity was determined by 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity (Sc%). Seventy-four percent of army cadets were classified as having excellent aerobic conditioning (53.9 ± 3.0 ml · kg(-) · min(-1) predicted VO2max from the Cooper test). The median serum concentration of all enzymes increased 12 hours after marching (T1: CK 675%; ALT 59%; AST 336%; AST/ALT ratio 85%; p = 0.001) and 48 hours after the end of training (T2: CK 878%; ALT 256%; AST 418%; AST/ALT ratio 180%; p = 0.001). Sc% was higher in T2 (31.1 ± 9.8%; p = 0.01) than in T0 (3.4% change). Maximal oxygen consumption (ml · kg(-1) · min(-1)) correlated negatively with CK (r = -0.25; p = 0.009) and ALT (r = -0.21; p = 0.03) serum levels, and positively with the change in Sc% (r = 0.22; p = 0.04) at T2. The results indicate that intense military training can cause liver and muscle injury and that aerobic conditioning can be considered as a protective factor for these injuries.
本研究旨在比较陆军学员在传统军事体能训练项目后肌肉和肝脏损伤的生化标志物以及总抗氧化能力，并将这些影响与有氧适能相关联。在训练开始前12小时（T0）、30公里行军后12小时（T1）以及军事训练结束后48小时（T2）对男性陆军学员（n = 87；年龄，20±2岁）进行评估。使用自动分析仪测量血清肌酸激酶（CK）、天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）水平。通过2,2-二苯基-1-苦基肼自由基清除活性（Sc%）测定总抗氧化能力。74%的陆军学员被归类为具有优秀的有氧适能（根据库珀测试预测的最大摄氧量为53.9±3.0 ml·kg⁻¹·min⁻¹）。行军后12小时（T1：CK 675%；ALT 59%；AST 336%；AST/ALT比值85%；p = 0.001）和训练结束后48小时（T2：CK 878%；ALT 256%；AST 418%；AST/ALT比值180%；p = 0.001），所有酶的血清浓度中位数均升高。T2时的Sc%（31.1±9.8%；p = 0.01）高于T0（变化3.4%）。最大耗氧量（ml·kg⁻¹·min⁻¹）与T2时的CK（r = -0.25；p = 0.009）和ALT（r = -0.21；p = 0.03）血清水平呈负相关，与Sc%的变化呈正相关（r = 0.22；p = 0.04）。结果表明，高强度军事训练可导致肝脏和肌肉损伤，有氧适能可被视为这些损伤的保护因素。
