Nomaguchi H, Matsuoka M, Kohsaka K, Nakata A, Ito T
Research Institute for Microbial Diseases, Osaka University, Japan.
Int J Lepr Other Mycobact Dis. 1989 Dec;57(4):817-24.
The 65-kDa protein of Mycobacterium leprae was produced in an Escherichia coli strain carrying a plasmid harboring the recloned gene coding for the protein. The protein was purified through affinity chromatography prepared with the IgG fraction of a monoclonal antibody which was prepared against the 65-kDa protein. The purified 65-kDa protein also reacted immunologically with the monoclonal antibody IIIE9, which recognizes the epitope for M. leprae, prepared by Buchanan, et al. BALB/c mice were inoculated with M. leprae and 4 months later were skin tested with the purified 65-kDa protein. Gross changes were observed at the skin-test site. The role of the protein in protective immunity against M. leprae foot pad infection in mice was also studied.
麻风分枝杆菌的65 kDa蛋白是在携带一种质粒的大肠杆菌菌株中产生的，该质粒含有编码该蛋白的重新克隆基因。该蛋白通过用针对65 kDa蛋白制备的单克隆抗体的IgG组分制备的亲和层析进行纯化。纯化的65 kDa蛋白也与布坎南等人制备的识别麻风分枝杆菌表位的单克隆抗体IIIE9发生免疫反应。用麻风分枝杆菌接种BALB/c小鼠，4个月后用纯化的65 kDa蛋白进行皮肤试验。在皮肤试验部位观察到明显变化。还研究了该蛋白在小鼠抗麻风分枝杆菌足垫感染的保护性免疫中的作用。
