Gelber R H, Hunter S W, Murray L P, Siu P, Tsang M, Brennan P J
Medical Research Institute of California Pacific Medical Center, San Franscisco, USA.
Lepr Rev. 1994 Sep;65(3):175-80.
It had previously been discovered that intradermal mouse vaccination with a protein fraction of Mycobacterium leprae (called soluble proteins) in Freund's incomplete adjuvant (FIA) resulted in consistent and long-lived protection against M. leprae multiplication from subsequent viable footpad challenges. In this study certain density-gradient subfractions of this soluble protein, but not others, in FIA afforded vaccine protection. The results of this study suggest which M. leprae proteins may be involved in protective immunity, particularly 1-3 kD, 10 kD, 65 kD, and those of higher molecular weight.
此前已发现，在弗氏不完全佐剂（FIA）中用麻风分枝杆菌的一种蛋白质组分（称为可溶性蛋白）对小鼠进行皮内接种，可对随后的活脚垫攻击产生持续且持久的保护，防止麻风分枝杆菌繁殖。在本研究中，该可溶性蛋白的某些密度梯度亚组分（而非其他亚组分）在FIA中提供了疫苗保护作用。本研究结果表明哪些麻风分枝杆菌蛋白可能参与保护性免疫，特别是1 - 3kD、10kD、65kD以及那些分子量更高的蛋白。
