Wu Zhexue, Shon Jong Cheol, Lee Doohyun, Park Kab-Tae, Park Chang Seo, Lee Taeho, Lee Hye Suk, Liu Kwang-Hyeon
BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, 80 Daehak-ro, Buk-gu, Daegu, 702-701, Republic of Korea.
Department of Chemical and Biochemical Engineering, Dongguk University, 26 Pil-dong 3-ga, Jung-gu, Seoul, 100-715, Republic of Korea.
Anal Bioanal Chem. 2016 Mar;408(8):2069-82. doi: 10.1007/s00216-015-9239-4. Epub 2016 Jan 27.
Skin ceramides are sphingolipids consisting of sphingoid bases, which are linked to fatty acids via an amide bond. Typical fatty acid acyl chains are composed of α-hydroxy fatty acid (A), esterified ω-hydroxy fatty acid (EO), non-hydroxy fatty acid (N), and ω-hydroxy fatty acid (O). We recently established a lipidomic platform to identify skin ceramides with non-hydroxyacyl chains using tandem mass spectrometry. We expanded our study to establish a lipidomic platform to identify skin ceramides with α-hydroxyacyl chains. Tandem mass spectrometry analysis of A-type ceramides using chip-based direct infusion nanoelectrospray-mass spectrometry showed the characteristic fragmentation pattern of both acyl and sphingoid units, which can be applied for structural identification of ceramides. Based on the tandem mass spectrometry fragmentation patterns of A-type ceramides, comprehensive fragmentation schemes were proposed. Our results may be useful for identifying A-type ceramides in the stratum corneum of human skin.
皮肤神经酰胺是由鞘氨醇碱组成的鞘脂类，鞘氨醇碱通过酰胺键与脂肪酸相连。典型的脂肪酸酰基链由α-羟基脂肪酸（A）、酯化ω-羟基脂肪酸（EO）、非羟基脂肪酸（N）和ω-羟基脂肪酸（O）组成。我们最近建立了一个脂质组学平台，用于使用串联质谱法鉴定具有非羟基酰基链的皮肤神经酰胺。我们扩展了研究，建立了一个脂质组学平台，用于鉴定具有α-羟基酰基链的皮肤神经酰胺。使用基于芯片的直接进样纳米电喷雾质谱对A型神经酰胺进行串联质谱分析，显示了酰基和鞘氨醇单元的特征性裂解模式，可用于神经酰胺的结构鉴定。基于A型神经酰胺的串联质谱裂解模式，提出了全面的裂解方案。我们的结果可能有助于鉴定人皮肤角质层中的A型神经酰胺。
