Park Kab-Tae, Shon Jong Cheol, Kim Ji-Eun, Park Gyu Hwan, Choi Hyun Jin, Liu Kwang-Hyeon
BK21 Plus KNU Multi-Omics Based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea.
College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Daegu, 41566, Korea.
Lipids. 2017 Feb;52(2):179-187. doi: 10.1007/s11745-016-4224-z. Epub 2017 Jan 11.
Lipid distribution in the brain is important for many biological functions and has been associated with some brain diseases. The aim of this study was to investigate lipid distribution in different regions of brain tissue in mice. To this end, substantia nigra (SN), caudate putamen (CPu), hippocampus (Hip), hypothalamus (Hyp), and cortex (Cx) tissues of mice were analyzed using direct infusion nanoelectrospray-ion trap mass spectrometry and multivariate analyses. The SN, CPu, Hip, Hyp, and Cx groups showed clear differences in lipid distribution using principal component analysis and a partial least-squares discriminant analysis score plot, and lipid levels were significantly different in different brain regions. In particular, sulfatides were mainly distributed in the SN region. Our results could be used to help understand the functions and mechanisms of lipids in various brain diseases.
脂质在大脑中的分布对许多生物学功能都很重要，并且与一些脑部疾病有关。本研究的目的是调查小鼠脑组织不同区域的脂质分布。为此，使用直接注入纳米电喷雾-离子阱质谱法和多变量分析对小鼠的黑质（SN）、尾状壳核（CPu）、海马体（Hip）、下丘脑（Hyp）和皮质（Cx）组织进行了分析。通过主成分分析和偏最小二乘判别分析得分图，SN、CPu、Hip、Hyp和Cx组在脂质分布上表现出明显差异，不同脑区的脂质水平也存在显著差异。特别是，硫脂主要分布在SN区域。我们的结果可用于帮助理解脂质在各种脑部疾病中的功能和机制。
