Skariyachan Sinosh, Prasanna Apoorva, Manjunath Sirisha P, Karanth Soujanya S, Nazre Ambika
Department of Biotechnology Engineering, Dayananda Sagar Institutions, Bangalore, 560078, India.
Visvesvaraya Technological University, Belagavi, Karnataka, India.
Environ Monit Assess. 2016 Feb;188(2):121. doi: 10.1007/s10661-016-5125-6. Epub 2016 Jan 27.
Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. is one of the edible mushrooms currently gaining attention as environmental restorer. The present study explores the potential of P. ostreatus (Jacq.: Fr.) P. Kumm. in degradation of textile dyes and effluents. The mushroom cultivation was carried out using paddy bed as substrate. The fully grown mushroom fruit bodies were used as a bioremediation agent against two industrially important azo dyes such as nylon blue and cotton yellow and few effluents collected from various textile industries in Karnataka, India. The ideal growth parameters such as temperature, pH, and dye concentrations for effective degradation were carried out. One of the main enzymes, laccase, responsible for biodegradation, was partially characterized. The degradation was found to be ideal at pH 3.0 and temperature at 26-28 °C. This study demonstrated a percentage degradation of 78.10, 90.81, 82.5, and 64.88 for dye samples such as nylon blue (50 ppm), cotton yellow (350 ppm), KSIC effluents, and Ramanagar effluents at 28 °C within 15th days respectively in comparison with other temperature conditions. Similarly, a percentage degradation of 35.99, 33.33, 76.13 and 25.8 for nylon blue (50 ppm), cotton yellow (350 ppm), Karnataka Silk Industries Corporation (KSIC) effluents and Ramnagar effluents were observed at pH 3.0 within 15 days, respectively (p < 0.05). Thus, the current study concluded that the utilization of P. ostreatus (Jacq.: Fr.) P. Kumm. at ideal environmental conditions is a cost-effective and eco-friendly approach for the degradation of various azo dyes and textile effluents which are harmful to the ecosystem.
糙皮侧耳（Pleurotus ostreatus (Jacq.: Fr.) P. Kumm.）是目前作为环境修复者而受到关注的食用蘑菇之一。本研究探讨了糙皮侧耳（Pleurotus ostreatus (Jacq.: Fr.) P. Kumm.）在降解纺织染料和废水方面的潜力。使用稻田床作为底物进行蘑菇栽培。将完全成熟的蘑菇子实体用作生物修复剂，用于处理两种具有工业重要性的偶氮染料，如尼龙蓝和棉黄，以及从印度卡纳塔克邦的各个纺织工业收集的少量废水。研究了有效降解的理想生长参数，如温度、pH值和染料浓度。对负责生物降解的主要酶之一漆酶进行了部分表征。发现降解在pH 3.0和温度26 - 28°C时最为理想。本研究表明，与其他温度条件相比，在28°C下，第15天时尼龙蓝（50 ppm）、棉黄（350 ppm）、KSIC废水和拉马纳加尔废水等染料样品的降解率分别为78.10%、90.81%、82.5%和64.88%。同样，在pH 3.0时，第15天时尼龙蓝（50 ppm）、棉黄（350 ppm）、卡纳塔克邦丝绸工业公司（KSIC）废水和拉马纳加尔废水的降解率分别为35.99%、33.33%、76.13%和25.8%（p < 0.05）。因此，本研究得出结论，在理想环境条件下利用糙皮侧耳（Pleurotus ostreatus (Jacq.: Fr.) P. Kumm.）是一种经济高效且生态友好的方法，可用于降解对生态系统有害的各种偶氮染料和纺织废水。
