Xu Qingcai, Dong Yuliang, Zhu Huayu, Sun Aide
Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China.
Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Chemistry and Chemical Engineering, Linyi University, Linyi 276005, China; State Key Laboratory of Isotope Geochemistry, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China.
Int J Anal Chem. 2015;2015:364242. doi: 10.1155/2015/364242. Epub 2015 Dec 24.
Knowledge of boron and its isotope in plants is useful to better understand the transposition and translocation of boron within plant, the geochemical behavior in the interface between soil and plant, and the biogeochemical cycle of boron. It is critical to develop a useful method to separate boron from the plant for the geochemical application of boron and its isotope. A method was developed for the extraction of boron in plant sample, whose isotope was determined by thermal ionization mass spectrometry. The results indicated that this method of dry ashing coupled with two-step ion-exchange chromatography is powerful for the separation of boron in plant sample with large amounts of organic matters completely. The ratios of boron isotope composition in those plant tissue samples ranged from -19.45‰ to +28.13‰ (total range: 47.58‰) with a mean value of 2.61 ± 11.76‰ SD. The stem and root isotopic compositions were lower than those in flower and leaf. The molecular mechanism of boron isotope may be responsible for the observed variation of boron isotopic composition and are considered as a useful tool for the better understanding of boron cycling process in the environment and for the signature of living systems.
了解植物中的硼及其同位素，有助于更好地理解硼在植物体内的转移和运输、土壤与植物界面的地球化学行为以及硼的生物地球化学循环。开发一种从植物中分离硼的有效方法，对于硼及其同位素的地球化学应用至关重要。本文开发了一种用于提取植物样品中硼的方法，并通过热电离质谱法测定其同位素。结果表明，这种干灰化结合两步离子交换色谱法，能够有效地完全分离含有大量有机物的植物样品中的硼。这些植物组织样品的硼同位素组成比值在-19.45‰至+28.13‰之间（总范围：47.58‰），平均值为2.61±11.76‰ SD。茎和根的同位素组成低于花和叶。硼同位素的分子机制可能是观察到的硼同位素组成变化的原因，被认为是更好地理解环境中硼循环过程和生物系统特征的有用工具。
