Popham Holly J R, Ellersieck Mark R, Li Huarong, Bonning Bryony C
USDA Agricultural Research Service, Biological Control of Insects Research Laboratory, Columbia, MO, USA.
AgBiTech, 1601 S. Providence Rd., Columbia, MO, 65211, USA.
Methods Mol Biol. 2016;1350:407-44. doi: 10.1007/978-1-4939-3043-2_21.
A considerable amount of work has been undertaken to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50 % of infected larvae (LC50 or LD50), median survival of larvae infected (ST50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and the corresponding data analyses for assessment of the insecticidal activity of baculovirus insecticides.
为了从基因层面提高杆状病毒杀虫剂的效力，人们已经开展了大量工作。构建出基因改造杆状病毒后，需通过实验室生物测定来量化杀虫活性的各项参数，比如杀死50%受感染幼虫所需的半数致死浓度（或剂量）（LC50或LD50）、受感染幼虫的半数存活时间（ST50），以及受感染幼虫造成的取食损害。本章介绍了用于评估杆状病毒杀虫剂杀虫活性的各种生物测定方法及相应的数据分析方法。
