Ouyang Nengtai, Wang Lin
Department of Pathology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 33 Yingfeng Road, Guangzhou, 510228, People's Republic of China.
Methods Mol Biol. 2016;1406:3-9. doi: 10.1007/978-1-4939-3444-7_1.
The in situ observation on the tissues, such as histopathology, immunohistochemistry (IHC), immunofluorescence (IF), and in situ hybridization (ISH), is one of the most important methods in the biomedical scientific research. In this chapter we introduce the most often used methods-hematoxylin and eosin (H&E) and double IF staining. H&E staining is used for general morphology by which the different pathological types of breast lesions are identified. The double IF staining is often used to study the protein-protein interaction on tissues for signaling mechanisms. This chapter also includes the histopathology of primary or simplified breast lesion types that is essential for applying the above methods and the reclassification of breast cancers by molecular markers.
对组织进行原位观察，如组织病理学、免疫组织化学（IHC）、免疫荧光（IF）和原位杂交（ISH），是生物医学科研中最重要的方法之一。在本章中，我们介绍最常用的方法——苏木精和伊红（H&E）染色及双重免疫荧光染色。H&E染色用于观察一般形态，以识别不同病理类型的乳腺病变。双重免疫荧光染色常用于研究组织上蛋白质 - 蛋白质相互作用的信号传导机制。本章还包括原发性或简化乳腺病变类型的组织病理学，这对于应用上述方法以及通过分子标志物对乳腺癌进行重新分类至关重要。
