Opt Express. 2021 Apr 26;29(9):13864-13875. doi: 10.1364/OE.423740.
Hematoxylin and eosin (H&E) staining is the gold standard for most histopathological diagnostics but requires lengthy processing times not suitable for point-of-care diagnosis. Here we demonstrate a 266-nm excitation ultraviolet photoacoustic remote sensing (UV-PARS) and 1310-nm microscopy system capable of virtual H&E 3D imaging of tissues. Virtual hematoxylin staining of nuclei is achieved with UV-PARS, while virtual eosin staining is achieved using the already implemented interrogation laser from UV-PARS for scattering contrast. We demonstrate the capabilities of this dual-contrast system for en-face planar and depth-resolved imaging of human tissue samples exhibiting high concordance with H&E staining procedures and confocal fluorescence microscopy. To our knowledge, this is the first microscopy approach capable of depth-resolved imaging of unstained thick tissues with virtual H&E contrast.
苏木精和伊红（H&E）染色是大多数组织病理学诊断的金标准，但需要冗长的处理时间，不适合即时诊断。在这里，我们展示了一种 266nm 激发的紫外光声远程传感（UV-PARS）和 1310nm 显微镜系统，能够对组织进行虚拟 H&E 三维成像。UV-PARS 实现了细胞核的虚拟苏木精染色，而虚拟伊红染色则利用已经实现的 UV-PARS 中的探测激光来实现散射对比。我们展示了这种双对比系统用于人组织样本的共面平面和深度分辨成像的能力，与 H&E 染色程序和共聚焦荧光显微镜高度一致。据我们所知，这是第一种能够对未染色的厚组织进行深度分辨成像并具有虚拟 H&E 对比的显微镜方法。
