Li Yinghui, Qiu Yaqun, Xian Huixia, Shi Xiaolu, Lin Yiman, Chen Qiongcheng, Jiang Min, Jiang Yixiang, Hu Lulu, Hu Qinghua
Shenzhen Key Laboratory of Priority Infectious Disease Control, Shenzhen Center for Disease Control and Prevention, Shenzhen 518055, China.
School of Life Sciences, Shenzhen University, Shenzhen 518060, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2016 Jan;37(1):115-8. doi: 10.3760/cma.j.issn.0254-6450.2016.01.025.
To understand the epidemiologic and etiologic characteristics of diarrheagenic Escherichia (E.) coli infections in Shenzhen.
Stool samples were collected from acute diarrheal patients in four sentinel hospitals in Shenzhen and diarrheagenic E. coli strains were isolated and identified with multiplex real-time PCR. Serotyping and pulsed-field gel electrophoresis (PFGE) typing were conducted for the diarrheagenic E. coli isolates.
A total of 74 diarrheagenic E. coli strains were isolated from 1 823 stool samples (4.06%). The patients were mainly young children aged <3 years and adults aged 20-39 years, and the infections mainly occurred during May-September of a year. Enterotoxigenic E. coli (ETEC) and enteropathognic E. coli (EPEC) were predominant (45.9% and 31.1%). Serogroups and PFGE patterns varied among the diarrheagenic E. coli isolates. However, serogroup O159 were predominant in ETEC and there were 5 clusters with ≥2 strains sharing same PFGE patterns.
ETEC and EPEC were predominant in diarrheagenic E. coli strains isolated from diarrheal patients in Shenzhen. Age and season specific characteristics of diarrheagenic E. coli infections were observed. The serotypes and PFGE patterns of diarrheagenic E. coli strains varied. Close attention should be paid to the possible ETEC outbreak.
了解深圳地区致泻性大肠埃希菌感染的流行病学及病原学特征。
收集深圳4家哨点医院急性腹泻患者的粪便样本，采用多重实时荧光定量PCR方法分离鉴定致泻性大肠埃希菌。对分离出的致泻性大肠埃希菌进行血清分型及脉冲场凝胶电泳（PFGE）分型。
从1823份粪便样本中分离出74株致泻性大肠埃希菌（4.06%）。患者以<3岁幼儿及2039岁成年人为主，感染主要发生在每年59月。产肠毒素大肠埃希菌（ETEC）和致病性大肠埃希菌（EPEC）为主（分别占45.9%和31.1%）。致泻性大肠埃希菌分离株的血清群及PFGE图谱存在差异。其中，ETEC中O159血清群为主，有5个聚类，≥2株具有相同的PFGE图谱。
深圳腹泻患者分离出的致泻性大肠埃希菌中ETEC和EPEC为主。观察到致泻性大肠埃希菌感染具有年龄及季节特异性特征。致泻性大肠埃希菌菌株的血清型及PFGE图谱存在差异。应密切关注ETEC可能出现的暴发情况。
