Yang Lulu, Luo Yan, Ma Lin, Wang Hong, Ling Wenwu, Li Jiawu, Qi Xiaoying, Lu Qiang, Chen Kefei
Department of Ultrasound, West China Hospital of Sichuan University, Chengdu, P.R. China.
Exp Anim. 2016 May 20;65(2):165-73. doi: 10.1538/expanim.15-0108. Epub 2016 Jan 28.
Liver transplantation may fail due to complications of insufficient portal vein (PV) flow such as portal vein stenosis (PVS). Therefore, establishing a model to explore the effect of PV flow on liver regeneration is crucial and essential. Rats were randomly divided into 6 groups: sham operation rats group; 70% partial hepatectomy (PH) group (group A); PVS groups with mild, moderate, or severe stenosis (group B-D) and portal vein ligation (PVL) group. PVS was produced by ligating PV with parallelly placed needles of different gauges. Ultrasound was performed to validate the stenosis ratio (SR) and velocity ratio (VR) at the prestenotic and stenotic site. Rats were sacrificed on day 1,3,7, and 14 after surgery, and liver regeneration rate (LRR) was calculated. We successfully established rat models of different degrees of PVS following 70%PH in 72 rats. The SRs of each PVS group were 44.8 ± 5.23%, 59.3 ± 4.07% and 69.5 ± 2.17%, which showed no statistical differences compared with those measured by stenosis ratio measured by ultrasound. The survival rate in groups A-D were 100%, 83.3%, 66.7% and 50% respectively. Differences were demonstrated between groups A and C, as well as groups A and D (both P<0.05). Moreover, LRR negatively correlated with SRu and VR, and the correlation coefficients were -0.534 and -0.522, respectively. The rat model we established has the potential to be applied in most conditions of liver regeneration with reduced PV inflow, and it provides a foundation for further exploring the relationship between PV hemodynamic changes and liver regeneration.
肝移植可能因门静脉（PV）血流不足的并发症（如门静脉狭窄，PVS）而失败。因此，建立一个模型来探究PV血流对肝再生的影响至关重要。将大鼠随机分为6组：假手术大鼠组；70%肝部分切除术（PH）组（A组）；轻度、中度或重度狭窄的PVS组（B - D组）和门静脉结扎（PVL）组。通过用不同规格的平行放置的针结扎PV来制造PVS。在狭窄前和狭窄部位进行超声检查以验证狭窄率（SR）和流速比（VR）。在术后第1、3、7和14天处死大鼠，并计算肝再生率（LRR）。我们成功地在72只大鼠中建立了70%PH后不同程度PVS的大鼠模型。各PVS组的SR分别为44.8±5.23%、59.3±4.07%和69.5±2.17%，与超声测量的狭窄率相比无统计学差异。A - D组的存活率分别为100%、83.3%、66.7%和50%。A组与C组以及A组与D组之间存在差异（均P<0.05）。此外，LRR与SRu和VR呈负相关，相关系数分别为 - 0.534和 - 0.522。我们建立的大鼠模型有可能应用于大多数PV流入减少的肝再生情况，并为进一步探究PV血流动力学变化与肝再生之间的关系提供了基础。
