Jiang Yue-Quan, Zhang Zhi, Cai Hua-Rong, Zhou Hong
Department of Thoracic Surgery, Chongqing Cancer Institute Chongqing, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):13785-94. eCollection 2015.
The killing effect of TNF mediated by conditionally replicating adenovirus SG502 on human cancer cell lines was assessed by in vivo and in vitro experiments.
The recombinant adenovirus SG502-TNF was used to infect human lung cancer cell line A549 and human esophageal cancer cell line TE-1. The expression of the exogenous gene and its inhibitory effect on the tumor cell lines were thus detected. Tumor transplantation experiment was performed in mice with the purpose of assessing the inhibitory effect of the adenovirus on tumor cells and tumor formation. The targeting of the adenovirus and the mechanism of tumor inhibition were discussed by in vivo imaging technology, HE staining and TUNEL assay.
Recombinant adenovirus SG502-TNF targeted the tumor cells specifically with stable expression of TNF, which produced a killing effect on tumor cells by regulating the apoptotic signaling pathway.
Recombinant adenovirus SG502-TNF possessed significant killing effect on TE-1 cells either in vivo or in vitro. This finding demonstrated the potential clinical application of adenovirus SG502.
通过体内和体外实验评估条件复制腺病毒SG502介导的肿瘤坏死因子（TNF）对人癌细胞系的杀伤作用。
用重组腺病毒SG502-TNF感染人肺癌细胞系A549和人食管癌细胞系TE-1。检测外源基因的表达及其对肿瘤细胞系的抑制作用。在小鼠中进行肿瘤移植实验，以评估腺病毒对肿瘤细胞和肿瘤形成的抑制作用。通过体内成像技术、苏木精-伊红（HE）染色和脱氧核糖核苷酸末端转移酶介导的缺口末端标记（TUNEL）法探讨腺病毒的靶向性及肿瘤抑制机制。
重组腺病毒SG502-TNF特异性靶向肿瘤细胞，TNF表达稳定，通过调节凋亡信号通路对肿瘤细胞产生杀伤作用。
重组腺病毒SG502-TNF在体内和体外对TE-1细胞均具有显著的杀伤作用。这一发现证明了腺病毒SG502潜在的临床应用价值。
