Mu Da-Wei, Guo He-Qing, Zhou Gao-Biao, Li Jian-Ye, Su Bin
Department of Urology, Air Force General Hospital Beijing 100142, China.
Int J Clin Exp Pathol. 2015 Nov 1;8(11):13864-70. eCollection 2015.
Oleanolic acid has significant pharmacological activities, such as anti-tumor, regulating blood sugar level and liver protection, which are more effective compared with free aglyconeoleanolic acid. However, it is still unknown if oleanolic acid affects the proliferation of human bladder cancer. We utilized T24 cells to study the effect of oleanolic acid on the proliferation and apoptosis of human bladder cancer. In this study, we found that the anti-cancer effect of oleanolic acid significantly suppressed cell proliferation and increased apoptosis and caspase-3 activity of T24 cells. Furthermore, Akt, mTOR and S6K protein expression was greatly inhibited in T24 cells under oleanolic acid treatment. Meanwhile, ERK1/2 of phosphorylation protein expression was significantly promoted by oleanolic acid treatment. Taken together, we provided evidences that oleanolic acid was Akt/mTOR/S6K and ERK1/2 signaling-targeting anti-tumor agent. These findings represent new evidences that oleanolic acid suppresses the proliferation of human bladder cancer by Akt/mTOR/S6K and ERK1/2 signaling, and oleanolic acid may be used to prevent human bladder cancer.
齐墩果酸具有显著的药理活性，如抗肿瘤、调节血糖水平和肝脏保护作用，与游离的齐墩果酸苷元相比，其效果更佳。然而，齐墩果酸是否影响人膀胱癌的增殖仍不清楚。我们利用T24细胞研究齐墩果酸对人膀胱癌增殖和凋亡的影响。在本研究中，我们发现齐墩果酸的抗癌作用显著抑制了T24细胞的增殖，增加了其凋亡和半胱天冬酶-3活性。此外，在齐墩果酸处理下，T24细胞中Akt、mTOR和S6K蛋白表达受到极大抑制。同时，齐墩果酸处理显著促进了磷酸化蛋白ERK1/2的表达。综上所述，我们提供了证据表明齐墩果酸是靶向Akt/mTOR/S6K和ERK1/2信号通路的抗肿瘤药物。这些发现代表了新的证据，即齐墩果酸通过Akt/mTOR/S6K和ERK1/2信号通路抑制人膀胱癌的增殖，并且齐墩果酸可能用于预防人膀胱癌。
