Okamura H, Bérod A, Julien J F, Geffard M, Kitahama K, Mallet J, Bobillier P
INSERM U171-CNRS UA1195, Centre Hospitalier Lyon-Sud, Pierre Bénite, France.
Neurosci Lett. 1989 Jul 31;102(2-3):131-6. doi: 10.1016/0304-3940(89)90067-0.
The existence of GABAergic neurons in the rat suprachiasmatic nucleus (SCN) was demonstrated by three specific markers; mRNA coding for glutamic acid decarboxylase (GAD) and visualized by in situ hybridization using a 35S-labelled cDNA probe, and GAD protein and GABA were identified by immunocytochemistry using specific antisera. In situ hybridization demonstrated well labelled GAD mRNA positive cells throughout SCN, and GABA and GAD immunoreactive cells showed similar distributions. These results indicate that GABA is a transmitter of a large portion of the SCN neuronal population.
通过三种特异性标志物证实了大鼠视交叉上核（SCN）中γ-氨基丁酸能神经元的存在；编码谷氨酸脱羧酶（GAD）的mRNA，通过使用35S标记的cDNA探针进行原位杂交来可视化，以及通过使用特异性抗血清的免疫细胞化学鉴定GAD蛋白和γ-氨基丁酸。原位杂交显示整个视交叉上核中GAD mRNA阳性细胞标记良好，γ-氨基丁酸和GAD免疫反应性细胞显示出相似的分布。这些结果表明γ-氨基丁酸是视交叉上核大部分神经元群体的一种神经递质。
