Department of Systems Biology, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8501, Japan.
Department of Life Science and Biotechnology, Faculty of Chemistry, Materials and Bioengineering, Kansai University, Suita 564-8680, Japan.
Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2308489120. doi: 10.1073/pnas.2308489120. Epub 2023 Oct 16.
The circadian clock is a biological timekeeping system that oscillates with a circa-24-h period, reset by environmental timing cues, especially light, to the 24-h day-night cycle. In mammals, a "central" clock in the hypothalamic suprachiasmatic nucleus (SCN) synchronizes "peripheral" clocks throughout the body to regulate behavior, metabolism, and physiology. A key feature of the clock's oscillation is resistance to abrupt perturbations, but the mechanisms underlying such robustness are not well understood. Here, we probe clock robustness to unexpected photic perturbation by measuring the speed of reentrainment of the murine locomotor rhythm after an abrupt advance of the light-dark cycle. Using an intersectional genetic approach, we implicate a critical role for arginine vasopressin pathways, both central within the SCN and peripheral from the anterior pituitary.
生物钟是一个生物计时系统，以大约 24 小时的周期振荡，并通过环境定时线索（尤其是光）重置为 24 小时的昼夜循环。在哺乳动物中，下丘脑视交叉上核（SCN）中的“中央”时钟使全身的“外周”时钟同步，以调节行为、代谢和生理机能。时钟振荡的一个关键特征是对突然的干扰具有抵抗力，但这种稳健性的机制尚不清楚。在这里，我们通过测量光暗周期突然提前后小鼠运动节律重新同步的速度来探测时钟对意外光干扰的稳健性。使用交叉遗传方法，我们暗示精氨酸加压素途径在中央 SCN 内和外周从垂体前叶均发挥关键作用。
