Galas Aleksander, Miszczyk Justyna
Department of Epidemiology, Jagiellonian University-Medical College, Krakow, Poland.
Department of Experimental Physics of Complex Systems, Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland.
PLoS One. 2016 Jan 29;11(1):e0147658. doi: 10.1371/journal.pone.0147658. eCollection 2016.
There is still an open question how to predict colorectal cancer risk before any morphological changes appear in the colon.
The purpose was to investigate aberrations in chromosomes 1, 2 and 4 in peripheral blood lymphocytes analyzed by fluorescence in situ hybridization technique as a tool to assess the likelihood of colorectal cancer.
A hospital-based case-control study included 20 colon cancer patients and 18 hospital-based controls. Information about potential covariates was collected by interview. The frequency of stable and unstable chromosome aberrations in chromosome 1, 2 and 4 was assessed by fluorescence in situ hybridization technique.
Colorectal cancer patients, as compared to controls, had a relatively higher frequency of chromosome 1 translocations (median: 3.5 versus 1.0 /1000 cells, p = 0.006), stable aberrations (3.8 versus 1.0 /1000 cells, p = 0.007) and total aberrations (p = 0.009). There were no differences observed for chromosomes 2 and 4. Our results showed an increase in the odds of having colon cancer by about 50-80% associated with an increase by 1/1000 cells in the number of chromosome 1 aberrations.
The results revealed that the frequency of chromosomal aberrations, especially translocations in chromosome 1, seems to be a promising method to show a colon cancer risk. Additionally, our study suggests the reasonableness of use of biomarkers such as chromosome 1 aberrations in peripheral blood lymphocytes in screening prevention programs for individuals at higher colon cancer risk to identify those who are at increased risk and require more frequent investigations, e.g. by sigmoidoscopy.
在结肠出现任何形态变化之前，如何预测结直肠癌风险仍是一个悬而未决的问题。
旨在研究通过荧光原位杂交技术分析外周血淋巴细胞中1号、2号和4号染色体的畸变情况，以此作为评估结直肠癌发生可能性的一种工具。
一项基于医院的病例对照研究纳入了20例结肠癌患者和18例医院对照。通过访谈收集有关潜在协变量的信息。采用荧光原位杂交技术评估1号、2号和4号染色体中稳定和不稳定染色体畸变的频率。
与对照组相比，结直肠癌患者的1号染色体易位频率相对较高（中位数：3.5对1.0/1000个细胞，p = 0.006）、稳定畸变频率（3.8对1.0/1000个细胞，p = 0.007）和总畸变频率（p = 0.009）。2号和4号染色体未观察到差异。我们的结果显示，1号染色体畸变数量每增加1/1000个细胞，患结肠癌的几率增加约50 - 80%。
结果表明，染色体畸变频率，尤其是1号染色体的易位，似乎是一种显示结直肠癌风险的有前景的方法。此外，我们的研究表明，在针对结直肠癌风险较高个体的筛查预防项目中，使用外周血淋巴细胞中1号染色体畸变等生物标志物是合理的，以识别那些风险增加且需要更频繁检查的个体，例如通过乙状结肠镜检查。
