Hoffman Benjamin, Martin Matthew, Brown Bryan N, Bonassar Lawrence J, Cheetham Jonathan
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York.
McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, U.S.A.
Laryngoscope. 2016 Oct;126(10):E325-31. doi: 10.1002/lary.25861. Epub 2016 Jan 30.
OBJECTIVES/HYPOTHESIS: The trachea is essential to respiratory function and is a mechanically and biochemically complex composite tissue. Tissue-engineering approaches to treat tracheal diseases require detailed knowledge of the native mechanical and biochemical properties of the trachea. Although the porcine trachea represents an excellent preclinical model, relevant mechanical and biochemical composition are incompletely characterized.
Experimental. The mechanical and biochemical properties of 12 intact porcine tracheas were determined to characterize their compliance, as well as the aggregate modulus, bidirectional elastic modulus, hydraulic permeability, and biochemical characteristics of individual cartilage rings.
Data demonstrate the glycosaminoglycan content of tracheal rings was (mean ± standard deviation) 190 ± 49 μg/mg. Hydroxyproline content was 8.2 ± 3.2 μg/mg, and DNA content was 1.3 ± 0.27 μg/mg, a four-fold difference between circumferential elastic modulus (5.6 ± 2.0 megapascal [MPa]) and longitudinal composite elastic modulus (1.1 ± 0.7 MPa, P < 0.0001) was also observed. Aggregate modulus (stiffness) of porcine tracheal rings was 1.30 ± 0.28 MPa, and inflationary compliance was 0.00472 ± 0.00188 cmH2 O(-1) .
This study presents a comprehensive characterization of the relevant biochemical and mechanical properties of porcine tracheal cartilage, which is considered an excellent candidate for xenogenic tracheal graft and a source for tissue-engineered tracheal reconstruction. The range of parameters characterized in this study agrees with those reported for hyaline cartilage of the airway in other species. These characteristics can be used as quantitative benchmarks for tissue-engineering approaches to treat tracheal disease.
NA. Laryngoscope, 126:E325-E331, 2016.
目的/假设：气管对呼吸功能至关重要，是一种机械和生化性质复杂的复合组织。治疗气管疾病的组织工程方法需要详细了解气管的天然机械和生化特性。尽管猪气管是一种优秀的临床前模型，但其相关的机械和生化组成尚未完全明确。
实验性研究。测定12个完整猪气管的机械和生化特性，以表征其顺应性以及单个软骨环的聚集模量、双向弹性模量、水力渗透性和生化特性。
数据表明气管环的糖胺聚糖含量为（平均值±标准差）190±49μg/mg。羟脯氨酸含量为8.2±3.2μg/mg，DNA含量为1.3±0.27μg/mg，还观察到周向弹性模量（5.6±2.0兆帕[MPa]）与纵向复合弹性模量（1.1±0.7MPa，P<0.0001）之间存在四倍差异。猪气管环的聚集模量（刚度）为1.30±0.28MPa，充气顺应性为0.00472±0.00188cmH2O-1。
本研究全面表征了猪气管软骨的相关生化和机械特性，猪气管软骨被认为是异种气管移植的优秀候选材料和组织工程气管重建的来源。本研究中表征的参数范围与其他物种气道透明软骨报道的参数一致。这些特性可作为治疗气管疾病的组织工程方法的定量基准。
无。《喉镜》，2016年，第126卷，E325-E331页。
