Tiwari Yash Vardhan, Jiang Zhao, Sun Yuhao, Du Fang, Rodriguez Pavel, Shen Qiang, Duong Timothy Q
Research Imaging Institute, University of Texas Health Science Center, 8403 Floyd Curl Dr, San Antonio, TX 78229, USA; Department of Biomedical Engineering, University of Texas, San Antonio, TX, USA.
Research Imaging Institute, University of Texas Health Science Center, 8403 Floyd Curl Dr, San Antonio, TX 78229, USA.
Brain Res. 2016 Mar 15;1635:121-9. doi: 10.1016/j.brainres.2016.01.030. Epub 2016 Jan 26.
In order to improve clinical trial design and translation of normobaric oxygen (NBO) treatment of ischemic stroke, NBO treatment parameters need to be better understood. This study investigated NBO treatment efficacy at two different stroke severities and two NBO treatment durations in rats. For the 60-min middle cerebral artery occlusion (MCAO), NBO treatment for 25 min and 150 min were studied. For the 90-min MCAO, NBO treatment for 55 min and 150 min were studied. Cerebral blood flow (CBF), apparent diffusion coefficients (ADC) and T2 MRI were acquired during occlusion prior to treatment, after reperfusion, and 48h after MCAO. The effects of NBO treatment on lesion volumes, and CBF, ADC and T2 of ischemic core, perfusion-diffusion mismatch and normal tissue were analyzed longitudinally. The major findings were: i) NBO treatment was effective in both groups of stroke severities, salvaging similar percentage of initial abnormal ADC tissue, and ii) NBO treatments continued after reperfusion were more beneficial than NBO treatment during occlusion alone for both MCAO groups. These findings underscore the importance of the effects of NBO duration and stroke severity on treatment outcomes.
为了改善常压氧（NBO）治疗缺血性中风的临床试验设计及转化，需要更好地了解NBO治疗参数。本研究调查了在两种不同中风严重程度及两种NBO治疗时长下，NBO治疗对大鼠的疗效。对于60分钟的大脑中动脉闭塞（MCAO），研究了25分钟和150分钟的NBO治疗。对于90分钟的MCAO，研究了55分钟和150分钟的NBO治疗。在治疗前的闭塞期间、再灌注后以及MCAO后48小时采集脑血流量（CBF）、表观扩散系数（ADC）和T2磁共振成像（MRI）。纵向分析了NBO治疗对病变体积以及缺血核心、灌注 - 扩散不匹配区域和正常组织的CBF、ADC及T2的影响。主要发现为：i）NBO治疗在两组中风严重程度中均有效，挽救了相似比例的初始异常ADC组织；ii）对于两个MCAO组，再灌注后持续的NBO治疗比仅在闭塞期间进行NBO治疗更有益。这些发现强调了NBO持续时间和中风严重程度对治疗结果影响的重要性。
