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通过抑制大肠杆菌中的DNA修复缺陷来克隆真核DNA糖基化酶修复基因。

Cloning a eukaryotic DNA glycosylase repair gene by the suppression of a DNA repair defect in Escherichia coli.

作者信息

Chen J, Derfler B, Maskati A, Samson L

机构信息

Harvard School of Public Health, Charles A. Dana Laboratory of Toxicology, Boston, MA 02115.

出版信息

Proc Natl Acad Sci U S A. 1989 Oct;86(20):7961-5. doi: 10.1073/pnas.86.20.7961.

DOI:10.1073/pnas.86.20.7961
PMID:2682633
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC298192/
Abstract

If eukaryotic genes could protect bacteria with defects in DNA repair, this effect could be exploited for the isolation of eukaryotic DNA repair genes. We have thus cloned a DNA repair gene from Saccharomyces cerevisiae that directs the synthesis of a DNA glycosylase that specifically releases 3-methyladenine from alkylated DNA and in so doing protects alkylation-sensitive Escherichia coli from killing by methylating agents. The cloned yeast gene was then used to generate a mutant strain of S. cerevisiae that carries a defect in the glycosylase gene and is extremely sensitive to DNA methylation. This approach may allow the isolation of a large number of eukaryotic DNA repair genes.

摘要

如果真核基因能够保护DNA修复存在缺陷的细菌，那么这种效应可用于分离真核DNA修复基因。因此，我们从酿酒酵母中克隆了一个DNA修复基因，该基因指导合成一种DNA糖基化酶，这种酶能特异性地从烷基化DNA中释放3-甲基腺嘌呤，从而保护对烷基化敏感的大肠杆菌免受甲基化剂的杀伤。然后，利用克隆的酵母基因构建了酿酒酵母的一个突变菌株，该菌株的糖基化酶基因存在缺陷，对DNA甲基化极其敏感。这种方法可能有助于分离大量真核DNA修复基因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/cf956553baf3/pnas00287-0312-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/b55a09702838/pnas00287-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/c7507fa18697/pnas00287-0310-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/fdb49e8a907b/pnas00287-0310-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/27323fbb38b1/pnas00287-0310-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/6cbafdb3e6d7/pnas00287-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/cf956553baf3/pnas00287-0312-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/b55a09702838/pnas00287-0310-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/c7507fa18697/pnas00287-0310-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/fdb49e8a907b/pnas00287-0310-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/27323fbb38b1/pnas00287-0310-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/6cbafdb3e6d7/pnas00287-0312-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a56e/298192/cf956553baf3/pnas00287-0312-b.jpg

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Adaptation to alkylation resistance involves the induction of a DNA glycosylase.对烷化抗性的适应涉及一种DNA糖基化酶的诱导。
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Induction of a DNA glycosylase for N-methylated purines is part of the adaptive response to alkylating agents.诱导一种针对N-甲基化嘌呤的DNA糖基化酶是对烷化剂适应性反应的一部分。
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Two differentially regulated mRNAs with different 5' ends encode secreted with intracellular forms of yeast invertase.
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Selective base excision repair of DNA damage by the non-base-flipping DNA glycosylase AlkC.DNA 糖苷酶 AlkC 通过非碱基翻转的方式选择性修复 DNA 损伤。
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