对2008 - 2012年澳大利亚百日咳博德特氏菌流行菌株的基因组剖析。
Genomic dissection of Australian Bordetella pertussis isolates from the 2008-2012 epidemic.
作者信息
Safarchi Azadeh, Octavia Sophie, Wu Sunny Z, Kaur Sandeep, Sintchenko Vitali, Gilbert Gwendolyn L, Wood Nicholas, McIntyre Peter, Marshall Helen, Keil Anthony D, Lan Ruiting
机构信息
School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, New South Wales, Australia.
Centre for Infectious Diseases and Microbiology - Public Health, Institute of Clinical Pathology and Medical Research, Pathology West, Westmead Hospital, New South Wales, Australia; Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, New South Wales, Australia.
出版信息
J Infect. 2016 Apr;72(4):468-77. doi: 10.1016/j.jinf.2016.01.005. Epub 2016 Jan 27.
OBJECTIVES
Despite high pertussis vaccination coverage, Australia experienced a prolonged epidemic in 2008-2012. The predominant Bordetella pertussis genotype harboured pertussis toxin promoter allele, ptxP3, and pertactin gene allele, prn2. The emergence and expansion of prn non-expressing isolates (Prn negative), were also observed. We aimed to investigate the microevolution and genomic diversity of epidemic B. pertussis isolates.
METHODS
We sequenced 22 B. pertussis isolates collected in 2008-2012 from two states of Australia which are geographically widely separated. Ten of the 22 were Prn negative isolates with three different modes of silencing of prn (prn::IS481F, prn::IS481R and prn::IS1002). Five pre-epidemic isolates were also sequenced for comparison.
RESULTS
Five single nucleotide polymorphisms were common in the epidemic isolates and differentiated them from pre-epidemic isolates. The Australian epidemic isolates can be divided into five lineages (EL1-EL5) with EL1 containing only Prn negative isolates. Comparison with global isolates showed that three lineages remained geographically and temporally distinct whereas two lineages mixed with isolates from 2012 UK outbreak.
CONCLUSION
Our results suggest significant diversification and the microevolution of B. pertussis within the 2008-2012 Australian epidemic.
目的
尽管百日咳疫苗接种覆盖率很高，但澳大利亚在2008 - 2012年经历了一场长期的疫情。主要的百日咳博德特氏菌基因型携带百日咳毒素启动子等位基因ptxP3和百日咳杆菌粘附素基因等位基因prn2。同时也观察到了不表达百日咳杆菌粘附素的分离株（Prn阴性）的出现和扩散。我们旨在研究疫情期间百日咳博德特氏菌分离株的微观进化和基因组多样性。
方法
我们对2008 - 2012年从澳大利亚两个地理上相距甚远的州收集的22株百日咳博德特氏菌分离株进行了测序。22株中有10株是Prn阴性分离株，具有三种不同的prn沉默模式（prn::IS481F、prn::IS481R和prn::IS1002）。还对5株疫情前的分离株进行了测序以作比较。
结果
5个单核苷酸多态性在疫情分离株中很常见，并将它们与疫情前的分离株区分开来。澳大利亚疫情分离株可分为5个谱系（EL1 - EL5），其中EL1仅包含Prn阴性分离株。与全球分离株的比较表明，三个谱系在地理和时间上仍然不同，而两个谱系与2012年英国疫情的分离株混合在一起。
结论
我们的结果表明，在2008 - 2012年澳大利亚疫情期间，百日咳博德特氏菌发生了显著的多样化和微观进化。
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