Urbanová Veronika, Bazalová Olga, Vaněčková Hanka, Dolezel David
Institute of Entomology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic.
Institute of Entomology, Biology Center, Czech Academy of Sciences, 37005 Ceske Budejovice, Czech Republic; Department of Molecular Biology, Faculty of Sciences, University of South Bohemia, 37005 Ceske Budejovice, Czech Republic.
Insect Biochem Mol Biol. 2016 Mar;70:184-90. doi: 10.1016/j.ibmb.2016.01.003. Epub 2016 Jan 28.
Adult reproductive diapause is characterized by lower behavioral activity, ceased reproduction and absence of juvenile hormone (JH). The role of JH receptor Methoprene-tolerant (Met) in female reproduction is well established; however, its function in male reproductive development and behavior is unclear. In the bean bug, Riptortus pedestris, circadian genes are essential for mediating photoperiodically-dependent growth of the male accessory glands (MAGs). The present study explores the role of circadian genes and JH receptor in male diapause in the linden bug, Pyrrhocoris apterus. These data indicate that circadian factors Clock, Cycle and Cry2 are responsible for photoperiod measurement, whereas Met and its partner protein Taiman participate in JH reception. Surprisingly, knockdown of the JH receptor neither lowered locomotor activity nor reduced mating behavior of males. These data suggest existence of a parallel, JH-independent or JH-upstream photoperiodic regulation of reproductive behavior.
成年生殖滞育的特征是行为活动减少、繁殖停止以及保幼激素（JH）缺失。保幼激素受体耐甲氧普烯（Met）在雌性生殖中的作用已得到充分证实；然而，其在雄性生殖发育和行为中的功能尚不清楚。在豆蝽中，昼夜节律基因对于介导雄性附腺（MAGs）的光周期依赖性生长至关重要。本研究探讨了昼夜节律基因和保幼激素受体在菩提树蝽雄性滞育中的作用。这些数据表明，昼夜节律因子Clock、Cycle和Cry2负责光周期测量，而Met及其伴侣蛋白Tai则参与保幼激素的接收。令人惊讶的是，保幼激素受体的敲低既没有降低雄性的运动活性，也没有减少其交配行为。这些数据表明存在一种平行的、不依赖保幼激素或保幼激素上游的生殖行为光周期调节。
