Kubáň Pavel, Boček Petr
Institute of Analytical Chemistry of the Czech Academy of Sciences, v. v. i., Veveří 97, CZ-60200 Brno, Czech Republic.
Institute of Analytical Chemistry of the Czech Academy of Sciences, v. v. i., Veveří 97, CZ-60200 Brno, Czech Republic.
Anal Chim Acta. 2016 Feb 18;908:113-20. doi: 10.1016/j.aca.2016.01.007. Epub 2016 Jan 11.
Micro-electromembrane extractions (μ-EMEs) across free liquid membranes (FLMs) were applied to simultaneous extractions of anions and cations. A transparent narrow-bore polymeric tubing was filled with adjacent plugs of μL volumes of aqueous and organic solutions, which formed a stable five-phase μ-EME system. For the simultaneous μ-EMEs of anions and cations, aqueous donor solution was the central phase, which was sandwiched between two organic FLMs and two aqueous acceptor solutions. On application of electric potential, anions and cations in the donor solution migrated across the two FLMs and into the two peripheral acceptor solutions in the direction of anode and cathode, respectively. Visual monitoring of anionic (tartrazine) and cationic (phenosafranine) dye confirmed their simultaneous μ-EMEs and their rapid (in less than 5 min) transfers into anolyte and catholyte, respectively. The concept of simultaneous μ-EMEs was further examined with selected model analytes; KClO4 was used for μ-EMEs of inorganic anions and cations and ibuprofen and procaine for μ-EMEs of acidic and basic drugs. Quantitative analyses of the resulting acceptor solutions were carried out by capillary electrophoresis with capacitively coupled contactless conductivity detection (CE-C(4)D). Good extraction recoveries (91-94%) and repeatability of peak areas (≤6.3%) were achieved for 5 min μ-EMEs of K(+) and ClO4(-). Extraction recoveries and repeatability of peak areas for 5 min μ-EMEs of ibuprofen and procaine were also satisfactory and ranged from 35 to 63% and 7.6 to 11.3%, respectively. Suitability of the presented micro-extraction procedure was further demonstrated on simultaneous μ-EMEs with subsequent CE-C(4)D of ibuprofen and procaine from undiluted human urine samples.
基于游离液膜（FLM）的微电膜萃取（μ-EME）被用于同时萃取阴离子和阳离子。一根透明的窄孔聚合物管中填充有微升体积的相邻水相和有机相溶液塞，形成了一个稳定的五相μ-EME系统。对于阴离子和阳离子的同时μ-EME，水相供体溶液为中心相，夹在两个有机FLM和两个水相受体溶液之间。施加电势时，供体溶液中的阴离子和阳离子分别沿阳极和阴极方向穿过两个FLM并进入两个外围受体溶液。对阴离子（柠檬黄）和阳离子（番红花红）染料的可视化监测证实了它们的同时μ-EME以及它们分别快速（不到5分钟）转移到阳极电解液和阴极电解液中。使用选定的模型分析物进一步研究了同时μ-EME的概念；KClO4用于无机阴离子和阳离子的μ-EME，布洛芬和普鲁卡因用于酸性和碱性药物的μ-EME。通过电容耦合无接触电导检测毛细管电泳（CE-C(4)D）对所得受体溶液进行定量分析。对于K(+)和ClO4(-)的5分钟μ-EME，实现了良好的萃取回收率（91-94%）和峰面积重复性（≤6.3%）。布洛芬和普鲁卡因的5分钟μ-EME的萃取回收率和峰面积重复性也令人满意，分别为35%至63%和7.6%至11.3%。从未稀释的人类尿液样品中同时进行μ-EME并随后进行CE-C(4)D分析布洛芬和普鲁卡因，进一步证明了所提出的微萃取方法的适用性。
