State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China; Graduate University of Chinese Academy of Sciences, Beijing 100190, China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China.
Bioresour Technol. 2016 Apr;205:142-52. doi: 10.1016/j.biortech.2016.01.037. Epub 2016 Jan 22.
Periodic peristalsis was used to release water constraint and increase high solids enzymatic hydrolysis efficiency. Glucan and xylan conversion in periodic peristalsis enzymatic hydrolysis (PPEH) at 21% solid loading increased by 5.2-6.4% and 6.8-8.8% compared with that in incubator shaker enzymatic hydrolysis (ISEH), respectively. Hydrolysis kinetics suggested that sugars conversion significantly increased within 24h in PPEH compared with ISEH. The peak height of main water pool increased by 7.7-43.1% within 24h in PPEH compared with ISEH. The increases in peak height of main water pool were consistent with the increases in glucan conversion. Submicroscopic particulates and macro granule residues contributed greatly to water constraint compared with glucose, xylose, ethanol, and Tween 80. Smaller particle size and longer residence time resulted in lower water constraint and facilitated the enzymatic hydrolysis performance. Periodic peristalsis was an effective method to reduce water constraint and increase high solids enzymatic hydrolysis efficiency.
周期性蠕动用于释放水约束并提高高固含量酶水解效率。在 21%固含量下,周期性蠕动酶水解(PPEH)中的葡聚糖和木聚糖转化率分别比摇床孵育酶水解(ISEH)提高了 5.2-6.4%和 6.8-8.8%。水解动力学表明,与 ISEH 相比,PPEH 中糖的转化率在 24 小时内显著增加。与 ISEH 相比,PPEH 中主要水相的峰高在 24 小时内增加了 7.7-43.1%。主要水相峰高的增加与葡聚糖转化率的增加一致。与葡萄糖、木糖、乙醇和吐温 80 相比,亚微观颗粒和大颗粒残渣对水约束的贡献更大。较小的粒径和较长的停留时间导致较低的水约束,并促进了酶水解性能。周期性蠕动是一种减少水约束和提高高固含量酶水解效率的有效方法。