Silva Camila Batista da, Groppo Francisco Carlos, Santos Cleiton Pita dos, Serpe Luciano, Franz-Montan Michelle, Paula Eneida de, Ranali José, Volpato Maria Cristina
Department of Physiological Sciences, Piracicaba Dental School, University of Campinas - UNICAMP, Piracicaba, São Paulo, Brazil. Av. Limeira, 901 - Bairro Areião CEP 13414-903 Piracicaba, São Paulo, Brazil.
Department of Biochemistry, Institute of Biology, University of Campinas - UNICAMP, Campinas, São Paulo, Brazil, Rua Monteiro Lobato, 255 - Campinas - SP - Brasil - CEP 13083-862.
Br J Oral Maxillofac Surg. 2016 Apr;54(3):295-300. doi: 10.1016/j.bjoms.2016.01.005. Epub 2016 Jan 27.
We compared the efficacy of articaine encapsulated in multilamellar and unilamellar liposomes with that of articaine with epinephrine, after infiltration into inflamed and uninflamed tissue in rats. We encapsulated 4% articaine in multilamellar (articaine:multi) and unilamellar (articaine:uni) liposomes and compared them with 4% articaine with 1:100 000 epinephrine (articaine:epinephrine), in inflamed (plantar incision into the hind paw) and uninflamed (infraorbital nerve block) tissue in rats. Anaesthetic formulations (0.1ml) were injected near the right infraorbital foramen in uninflamed tissue, where success and duration of anaesthesia were assessed by pinching the upper lip every 5 minutes. For inflamed tissue the anaesthetic formulations (0.1ml) were injected laterally into a surgical wound made 24 hours earlier in the plantar region of the rat's right hind paw. The degree of anaesthesia was assessed by application of forces laterally to the wound with electronic von Frey filaments. Articaine:uni resulted in less successful anaesthesia than both articaine:multi (p=1.1x10(-5)) and articaine:epinephrine (p=4.3x10(-8)) in uninflamed tissue, but there were no differences in duration or success of anaesthesia between articaine:epinephrine and articaine:multi. In inflamed tissue articaine:epinephrine gave significantly more effective anaesthesia for longer than articaine:uni (p=2.3x10(-6)), and articaine:epinephrine (p=1.8x10(-6)) formulations, which did not differ from each other. Multilamellar liposomal articaine could be an option for local anaesthesia in uninflamed tissues. However, articaine with epinephrine gave better results than liposomal formulations in inflamed tissue.
我们将多层和单层脂质体包裹的阿替卡因与含肾上腺素的阿替卡因的疗效进行了比较，这些药物被注入大鼠的炎症组织和非炎症组织中。我们将4%的阿替卡因包裹在多层（阿替卡因：多层）和单层（阿替卡因：单层）脂质体中，并将它们与含1:100 000肾上腺素的4%阿替卡因（阿替卡因：肾上腺素）进行比较，分别注入大鼠的炎症组织（后爪足底切开）和非炎症组织（眶下神经阻滞）中。将麻醉制剂（0.1毫升）注入非炎症组织的右侧眶下孔附近，每5分钟捏上唇来评估麻醉的成功率和持续时间。对于炎症组织，将麻醉制剂（0.1毫升）注入大鼠右后爪足底区域24小时前形成的手术伤口外侧。用电子von Frey细丝向伤口外侧施加力来评估麻醉程度。在非炎症组织中，阿替卡因：单层导致的麻醉成功率低于阿替卡因：多层（p = 1.1×10⁻⁵）和阿替卡因：肾上腺素（p = 4.3×10⁻⁸），但阿替卡因：肾上腺素和阿替卡因：多层之间在麻醉持续时间或成功率上没有差异。在炎症组织中，阿替卡因：肾上腺素比阿替卡因：单层产生的麻醉效果显著更有效且持续时间更长（p = 2.3×10⁻⁶），并且阿替卡因：肾上腺素制剂之间没有差异（p = 1.8×10⁻⁶）。多层脂质体阿替卡因可能是用于非炎症组织局部麻醉的一种选择。然而，在炎症组织中，含肾上腺素的阿替卡因比脂质体制剂效果更好。
