Marão Heloisa Fonseca, Mao Jeremy J, Casatti Cláudio Aparecido, Coelho Paulo G, Ervolino Edilson, Zhou Jian, da Silva Vanessa Ferreira, Panzarini Sônia Regina
Department of Surgery and Integrated Clinics, Aracatuba Dental School, Univ Estadual Paulista-UNESP, Rua José Bonifácio, 1193-Vila Mendonça, Aracatuba, SP 16015-050, Brazil.
Center for Craniofacial Regeneration, College of Dental Medicine, Columbia University, 630 West 168th St., New York, NY 10032, USA.
Arch Oral Biol. 2016 May;65:9-16. doi: 10.1016/j.archoralbio.2016.01.006. Epub 2016 Jan 30.
The aim of the study was to analyze the effects of ovariectomy on periodontal tissues following immediate tooth replantation by histomorphometric, immunohistochemistry, and μCT analysis.
Eighty wistar rats (Rattus norvegicus albinos) with normal estrous cycles were randomly divided into two groups: ovariectomized (OVX) and Sham. Two months after surgery, the rats' upper right incisor was extracted followed by immediate reimplantation. The animals were sacrificed after 28, 45, and 60 days healing time. Histomorphometric and immunohistochemical analysis were performed by evaluation of PCNA and TRAP straining.
The periodontal ligament was reinserted into the bone and cementum in the both groups. The immunohistochemical analysis revealed PCNA positive cells on the periodontal ligament in both groups at 28 days. Root resorption was noted at 45 days with immunoreactive cells for TRAP present in bone and tooth surface however no statistical differences between the groups were noticed. Histomorphometric analysis showed significant difference between groups in the periodontal ligament and root resorption parameters for the sub-items: intensity of chronic inflammatory infiltrate at 60 days (p<0.01), the organization of the periodontal ligament at 28 days (p<0.05), depth of root resorption at 45 days (p<0.05) and at 60 days (p<0.001). The μCT analysis showed multiple areas of bone resorption in association with OVX at 28 and 60 days with no significant differences between times in vivo.
The ovariectomy did not have significant influence in periodontal tissue parameters following tooth reimplantation.
本研究旨在通过组织形态计量学、免疫组织化学和μCT分析，分析卵巢切除对即刻牙再植后牙周组织的影响。
80只具有正常发情周期的Wistar大鼠（白化挪威大鼠）被随机分为两组：卵巢切除组（OVX）和假手术组。术后两个月，拔除大鼠右上颌切牙并即刻再植。在愈合28、45和60天后处死动物。通过评估PCNA和TRAP染色进行组织形态计量学和免疫组织化学分析。
两组的牙周膜均重新附着于骨和牙骨质。免疫组织化学分析显示，两组在28天时牙周膜上均有PCNA阳性细胞。在45天时观察到牙根吸收，骨和牙表面存在TRAP免疫反应性细胞，但两组之间未发现统计学差异。组织形态计量学分析显示，两组在牙周膜和牙根吸收参数的以下子项目上存在显著差异：60天时慢性炎症浸润强度（p<0.01）、28天时牙周膜的组织结构（p<0.05）、45天时牙根吸收深度（p<0.05）和60天时牙根吸收深度（p<0.001）。μCT分析显示，在28天和60天时，与卵巢切除相关的多个骨吸收区域，体内不同时间之间无显著差异。
卵巢切除对牙再植后的牙周组织参数没有显著影响。
