Yazdannejat H, Hosseinimehr S J, Ghasemi A, Pourfallah T A, Rafiei A
Mazandaran University of Medical Sciences Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center Sari Iran.
Mazandaran University of Medical Sciences Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center Sari Iran
Cell Mol Biol (Noisy-le-grand). 2016 Jan 11;62(1):30-3.
Losartan is an angiotensin II receptor (AT-II-R) blocker that is widely used by human for blood pressure regulation. Also, it has antitumor property. In this study, we investigated the radiosensitizing effect of losartan on cellular toxicity induced by ionizing radiation on prostate cancer and non-malignant fibroblast cells. Human prostate cancer (DU-145) and human non-malignant fibroblast cells (HFFF2) were treated with losartan at different concentrations (0.5, 1, 10, 50 and 100 µM) and then these cells were exposed to ionizing radiation. The cell proliferation was determined using MTT assay. Our results showed that losartan exhibited antitumor effect on prostate cancer cells; it was reduced cell survival to 66% at concentration 1 µM. Losartan showed an additive killing effect in combination with ionizing radiation on prostate cancer cell. The cell proliferation was reduced to 54% in the prostate cancer cells treated with losartan at concentration 1 µM in combination with ionizing radiation. Losartan did not exhibit any toxicity on HFFF2 cell. This result shows a promising effect of losartan on enhancement of therapeutic effect of ionizing radiation in patients during therapy.
氯沙坦是一种血管紧张素II受体（AT-II-R）阻滞剂，被广泛用于人体血压调节。此外，它还具有抗肿瘤特性。在本研究中，我们调查了氯沙坦对电离辐射诱导的前列腺癌和非恶性成纤维细胞毒性的放射增敏作用。用不同浓度（0.5、1、10、50和100 μM）的氯沙坦处理人前列腺癌（DU-145）和人非恶性成纤维细胞（HFFF2），然后将这些细胞暴露于电离辐射。使用MTT法测定细胞增殖。我们的结果表明，氯沙坦对前列腺癌细胞具有抗肿瘤作用；在1 μM浓度下，它将细胞存活率降低到了66%。氯沙坦与电离辐射联合对前列腺癌细胞显示出相加杀伤作用。在1 μM浓度的氯沙坦与电离辐射联合处理的前列腺癌细胞中，细胞增殖降低到了54%。氯沙坦对HFFF2细胞未表现出任何毒性。这一结果表明氯沙坦在治疗期间对增强患者电离辐射治疗效果具有良好作用。
