Department of Radiopharmacy, Faculty of Pharmacy, Pharmaceutical Sciences Research Center, Mazandaran University of Medical Sciences, Sari, Iran.
Department of Radiology and Radiation Oncology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.
J Bioenerg Biomembr. 2019 Jun;51(3):249-257. doi: 10.1007/s10863-019-09792-w. Epub 2019 Mar 7.
Despite radiotherapy is an effective regimen in cancer treatment, resistance to tumor therapy still is a major challenge to radiotherapy and results in cancer recurrence and metastasis. Then the sensitization of tumor cells to ionizing radiation (IR) would be beneficial in cancer treatment. The aim of this study was to evaluate the synergistic effect of mefenamic acid (MEF) on colon cancer cell (HT-29) exposure to IR. HT-29 cells were treated with MEF and then exposed to IR. The synergistic effect of MEF is evaluated by clonogenic assay and flow cytometry. The productions of reactive oxygen species (ROS) were determined in irradiated and treated cells with MEF. The findings of this study showed that MEF had anti-cancer effect on colon cancer cell line and it increased the apoptosis in irradiated HT-29 cells. Also MEF reduced the number of cell colonies when HT-29 cells pre-treated with MEF and irradiated. MEF increased ROS production in irradiated cells. This additive effect of MEF with IR in killing of HT-29 cell was observed at low (10 μM) and medium (100 μM) concentrations of MEF. The present study demonstrates that MEF to be an additive effect on apoptosis and cell death induced by IR in colon cancer cells.
尽管放疗是癌症治疗的有效方案，但肿瘤治疗的耐药性仍然是放疗的主要挑战，导致癌症复发和转移。因此，肿瘤细胞对电离辐射（IR）的敏感性增加将有利于癌症治疗。本研究旨在评估甲芬那酸（MEF）对结肠癌细胞（HT-29）暴露于 IR 时的协同作用。用 MEF 处理 HT-29 细胞，然后用 IR 照射。通过集落形成实验和流式细胞术评估 MEF 的协同作用。用 MEF 处理照射和处理的细胞，测定活性氧（ROS）的产生。本研究结果表明，MEF 对结肠癌细胞系具有抗癌作用，它增加了照射 HT-29 细胞的细胞凋亡。此外，当用 MEF 预处理 HT-29 细胞并照射时，MEF 减少了细胞集落的数量。MEF 增加了照射细胞中的 ROS 产生。在 MEF 的低（10 μM）和中（100 μM）浓度下，观察到 MEF 与 IR 在杀伤 HT-29 细胞方面的这种附加效应。本研究表明，MEF 对 IR 诱导的结肠癌细胞凋亡和细胞死亡具有附加作用。
