Monteiro C, Proença P, Tavares C, Castañera A, Corte Real F
National Institute of Legal Medicine and Forensic Sciences, Centre Branch, Largo da Sé Nova, Coimbra 3000-213, Portugal; CENCIFOR, Forensic Sciences Centre, Largo da Sé Nova, Coimbra 3000-213, Portugal.
National Institute of Legal Medicine and Forensic Sciences, Centre Branch, Largo da Sé Nova, Coimbra 3000-213, Portugal; CENCIFOR, Forensic Sciences Centre, Largo da Sé Nova, Coimbra 3000-213, Portugal.
Forensic Sci Int. 2016 Aug;265:65-9. doi: 10.1016/j.forsciint.2016.01.010. Epub 2016 Jan 16.
One of the techniques most widely used in ethanol analysis in forensic laboratories is undoubtedly the headspace gas chromatography with flame-ionization detection (HS-GC-FID) since the determination of this substance is carried out directly, without the need for additional purification procedures, which leads to increased productivity. This is a very important factor due to the high number of alcohol analysis requested to these laboratories. The presence of other volatile substances can cause a problem given the fact that they can be interferents in ethanol analysis by HS-GC-FID, which can have legal consequences related with driving under the influence of alcohol. The authors report a case of a routine analysis by HS-GC-FID for the determination of ethanol of a driver who has suffered an accident in which the use of two chromatographic columns with different polarities was essential to obtain an unequivocally identification of this substance in presence of an interfering volatile anesthetic administered in the hospital. The method was validated according to international recommendations before being introduced into routine laboratory in terms of selectivity, limits of detection (LOD), limits of quantification (LOQ), linearity, repeatability, intermediate precision, accuracy, robustness and carryover.
在法医实验室中，乙醇分析最广泛使用的技术之一无疑是顶空气相色谱 - 火焰离子化检测法（HS - GC - FID），因为该物质的测定是直接进行的，无需额外的纯化程序，这提高了工作效率。鉴于这些实验室收到的酒精分析数量众多，这是一个非常重要的因素。其他挥发性物质的存在可能会引发问题，因为它们可能会干扰HS - GC - FID对乙醇的分析，这可能会导致与酒后驾车相关的法律后果。作者报告了一个通过HS - GC - FID对一名发生事故的司机进行乙醇测定的常规分析案例，在该案例中，使用两根不同极性的色谱柱对于在医院施用的干扰性挥发性麻醉剂存在的情况下明确鉴定该物质至关重要。该方法在引入常规实验室之前，根据国际建议在选择性、检测限（LOD）、定量限（LOQ）、线性、重复性、中间精密度、准确性、稳健性和残留方面进行了验证。
