Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.
Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia.
Food Chem. 2016 Jun 1;200:159-66. doi: 10.1016/j.foodchem.2016.01.040. Epub 2016 Jan 11.
Conventional extraction techniques for plant phenolics are usually associated with high organic solvent consumption and long extraction times. In order to establish an environmentally friendly extraction method for grape skin phenolics, deep eutectic solvents (DES) as a green alternative to conventional solvents coupled with highly efficient microwave-assisted and ultrasound-assisted extraction methods (MAE and UAE, respectively) have been considered. Initially, screening of five different DES for proposed extraction was performed and choline chloride-based DES containing oxalic acid as a hydrogen bond donor with 25% of water was selected as the most promising one, resulting in more effective extraction of grape skin phenolic compounds compared to conventional solvents. Additionally, in our study, UAE proved to be the best extraction method with extraction efficiency superior to both MAE and conventional extraction method. The knowledge acquired in this study will contribute to further DES implementation in extraction of biologically active compounds from various plant sources.
传统的植物酚类物质提取技术通常需要大量有机溶剂和较长的提取时间。为了建立一种环保的葡萄皮酚类物质提取方法，我们考虑使用深共晶溶剂（DES）替代传统溶剂，并结合高效的微波辅助提取法（MAE）和超声辅助提取法（UAE）。首先，我们对五种不同的 DES 进行了筛选，发现以胆碱氯化物为基础、含有草酸作为氢键供体且含水量为 25%的 DES 最有前景，与传统溶剂相比，该 DES 能更有效地提取葡萄皮中的酚类化合物。此外，在我们的研究中，UAE 被证明是最好的提取方法，其提取效率优于 MAE 和传统提取方法。本研究获得的知识将有助于进一步将 DES 应用于从各种植物源中提取生物活性化合物。
