利用NRPSsp对非核糖体肽合成酶的表征

Characterization of Nonribosomal Peptide Synthetases with NRPSsp.

作者信息

Prieto Carlos

机构信息

Bioinformatics Service, Nucleus, University of Salamanca (USAL), Edificio I+D+i, C/ Espejo 2, Salamanca, 37007, Spain.

出版信息

Methods Mol Biol. 2016;1401:273-8. doi: 10.1007/978-1-4939-3375-4_17.

DOI:10.1007/978-1-4939-3375-4_17

PMID:26831714

Abstract

Bioinformatic sequence analysis allows the functional characterization of newly sequenced proteins. Nonribosomal peptide synthetases (NRPSs) are multi-modular enzymes involved in the biosynthesis of natural products. The current omics era has enabled the exponential growth of the sequenced NRPS, and it is important to characterize the final product of these synthetases. Here, how to achieve the prediction of substrates which bind to adenylation domains in NRPS with NRPSsp (www.nrpssp.com) bioinformatic tool is described.

摘要

生物信息序列分析可对新测序的蛋白质进行功能表征。非核糖体肽合成酶（NRPSs）是参与天然产物生物合成的多模块酶。当前的组学时代使得已测序的NRPS呈指数级增长，表征这些合成酶的最终产物很重要。本文描述了如何使用NRPSsp（www.nrpssp.com）生物信息工具来预测与NRPS中的腺苷化结构域结合的底物。