Lee Jiyeon, Lee Sujung, Chen Cheng, Shim Hyeran, Kim-Ha Jeongsil
Department of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Gwangjin-gu, Seoul, South Korea.
FEBS Lett. 2016 Feb;590(4):431-44. doi: 10.1002/1873-3468.12086. Epub 2016 Feb 15.
The Drosophila mid-oogenesis stages are notable as the time when most maternal mRNAs become localized at discrete regions of the oocyte. Microtubule rearrangement occurs during this period and is critical for the localization of axis-determining maternal mRNAs. We have identified shot as a key player in establishing the cytoskeletal arrangement required for the spatial localization of axis-determining maternal mRNAs. We also found that the spatial distribution of the Shot protein is regulated by its mRNA localization. Our results suggest that the RNA localization mechanism is used not only for restricted accumulation of patterning molecules but also for the microtubule organization that leads to the initial development of oocyte polarity.
果蝇卵子发生中期阶段值得注意，因为此时大多数母体mRNA定位于卵母细胞的离散区域。在此期间会发生微管重排，这对于确定轴的母体mRNA的定位至关重要。我们已确定Shot是建立确定轴的母体mRNA空间定位所需的细胞骨架排列的关键因素。我们还发现Shot蛋白的空间分布受其mRNA定位的调节。我们的结果表明，RNA定位机制不仅用于模式分子的受限积累，还用于导致卵母细胞极性初步发育的微管组织。
