Zhang Huai-Rong, Wang Yin-Zhu, Zhao Wei, Xu Jing-Juan, Chen Hong-Yuan
State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University , Nanjing 210023, China.
Shandong Province Key Laboratory of Detection Technology for Tumor Makers, College of Chemistry and Chemical Engineering, Linyi University , Linyi 276005, China.
Anal Chem. 2016 Mar 1;88(5):2884-90. doi: 10.1021/acs.analchem.5b04716. Epub 2016 Feb 12.
In this work, we developed a visual electrochemiluminescence (ECL) sensing platform based on a dual-bipolar electrode (D-BPE) array chip. The chip was composed of two arrays of BPEs and three separated arrays of reservoirs filled with buffer, Ru(bpy)3(2+)-TPrA and luminol solutions, respectively. Both BPEs served as ECL reporting platforms. By applying 6.0 V voltage, an array of orange electrochemiluminescence (ECL) signals belonging to Ru(bpy)3(2+) turned on. After adding DNAzyme and H2O2 in Ru(bpy)3(2+) and luminol reservoirs, the orange Ru(bpy)3(2+) signals decreased until vanished due to the quenching effect; meanwhile, a new array of blue ECL signals turned on because of the luminol-H2O2 ECL reaction. The designed D-BPE owns superior properties compared with the three-electrode system benefiting from the quantitative relation of bipolar systems, which greatly enhanced the ECL detection sensitivity. Meanwhile, the visual color-switch ECL and the ratiometric detecting principle made the results easier to evaluate and more accurate. Quantitative detection of HL-60 cancer cells from 320 cells/mL to 2.5 × 10(5) cells/mL with two linear ranges was achieved. The detection limit was down to 80 cells/mL. Finally, this D-BPE chip could distinguish the tumor cells from normal cells and provide a prospect for cancer diagnosis in a high-throughput, visual way.
在这项工作中，我们基于双极电极（D - BPE）阵列芯片开发了一种可视化电化学发光（ECL）传感平台。该芯片由两排BPE阵列以及三个分别装有缓冲液、Ru(bpy)3(2 +)-TPrA和鲁米诺溶液的独立储液器阵列组成。两个BPE均用作ECL报告平台。施加6.0 V电压后，一排属于Ru(bpy)3(2 +)的橙色电化学发光（ECL）信号开启。在Ru(bpy)3(2 +)和鲁米诺储液器中加入DNAzyme和H2O2后，由于猝灭效应，橙色的Ru(bpy)3(2 +)信号减弱直至消失；与此同时，由于鲁米诺 - H2O2 ECL反应，一排新的蓝色ECL信号开启。与三电极系统相比，所设计的D - BPE具有优异的性能，这得益于双极系统的定量关系，极大地提高了ECL检测灵敏度。同时，可视化的颜色切换ECL和比率检测原理使结果更易于评估且更准确。实现了对HL - 60癌细胞在320个细胞/毫升至2.5×10(5)个细胞/毫升范围内的两个线性区间的定量检测。检测限低至80个细胞/毫升。最后，这种D - BPE芯片能够区分肿瘤细胞和正常细胞，并为高通量、可视化的癌症诊断提供了前景。
