Hiramoto Kaoru, Villani Elena, Iwama Tomoki, Komatsu Keika, Inagi Shinsuke, Inoue Kumi Y, Nashimoto Yuji, Ino Kosuke, Shiku Hitoshi
Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
Department of Chemical Science and Engineering, School of Materials and Chemical Technology, Tokyo Institute of Technology, Yokohama 226-8502, Japan.
Micromachines (Basel). 2020 May 22;11(5):530. doi: 10.3390/mi11050530.
Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devices for ECL cell assays is introduced. To conclude, the direction of research in this field and its application prospects are described.
在基础研究和临床应用中，哺乳动物细胞分析都至关重要。在可用于细胞分析的各种技术中，电化学发光（ECL）因其融合了电化学和光谱学方法而备受关注。在本综述中，我们总结了用于哺乳动物细胞分析的基于ECL的系统的最新进展。综述首先总结了发光体的发展，这些发展为生物样品的ECL应用打开了大门。其次，介绍了基于ECL的成像系统，这是一种用于可视化单细胞形态和细胞内分子的新兴技术。在随后的部分中，总结了过去十年中开发的ECL传感器，其使用使得对细胞衍生分子的高灵敏度检测成为可能。尽管ECL免疫分析在商业应用方面已经很成熟，但在单细胞水平上检测生物分子仍然是一个挑战。因此，重点放在了直接从小部分细胞甚至单个细胞中检测细胞分子的ECL传感器上。最后，介绍了用于ECL细胞分析的双极电极装置。总之，描述了该领域的研究方向及其应用前景。
