McClosky Daniel D, Wang Bo, Chen Gong, Anderson Charles T
Department of Biology, The Pennsylvania State University, University Park, PA 16802, USA; Center for Lignocellulose Structure and Formation, The Pennsylvania State University, University Park, PA 16802, USA.
Center for Lignocellulose Structure and Formation, The Pennsylvania State University, University Park, PA 16802, USA; Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA.
Phytochemistry. 2016 Mar;123:16-24. doi: 10.1016/j.phytochem.2016.01.007. Epub 2016 Jan 28.
Plant cell walls are dynamic structures whose polysaccharide components are rearranged and recycled during growth and morphogenesis. Covalent fluorescent tagging of these polysaccharides following a metabolic labeling approach can help elucidate these changes. Herein reported are the synthesis and seedling-incorporation of a plant polysaccharide chemical reporter, 6-deoxy-alkynyl glucose (6dAG), that is modeled on D-glucose. Whereas fucose-alkyne, a previously reported chemical reporter for pectin, incorporates diffusely throughout growing cell walls, 6dAG incorporated specifically into root hair tips. This incorporation occurs in a time- and concentration-dependent manner. 6dAG exposure both induces and colocalizes with callose deposition in this tissue, and arrests both root hair and root growth. These results show that plants can incorporate an additional alkynyl-modified sugar analog into their metabolism, and into a discrete subcellular location.
植物细胞壁是动态结构，其多糖成分在生长和形态发生过程中会重新排列和循环利用。采用代谢标记方法对这些多糖进行共价荧光标记有助于阐明这些变化。本文报道了一种基于D-葡萄糖构建的植物多糖化学报告分子6-脱氧-炔基葡萄糖（6dAG）的合成及其在幼苗中的掺入情况。之前报道的用于果胶的化学报告分子岩藻糖-炔烃会扩散掺入整个生长中的细胞壁，而6dAG则特异性地掺入根毛尖端。这种掺入以时间和浓度依赖的方式发生。6dAG处理既诱导该组织中胼胝质沉积并与其共定位，又会抑制根毛和根的生长。这些结果表明，植物能够将另一种炔基修饰的糖类似物纳入其代谢过程，并使其进入特定的亚细胞位置。
