鼠疫耶尔森氏菌抗原的磁性免疫酶分析
[Magnetic immunoenzyme analysis of Yersinia pestis antigens].
作者信息
Klimova I M, Efremenko V I, Pushkar' V G
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1989 Jul(7):62-6.
PMID:2683524
Abstract
The detection of Y. pestis cells in magnetic enzyme immunoassay is carried out with the use of magnetic polyacrylamide microgranules. In the assay system for the determination of the antigen commercial Y. pestis antigens, peroxidase-labeled antibodies, the substrate mixture consisting of sodium salt of 2,2-azino-bis-3-ethylbenzthiazoline-6-sulfonic acid and H2O2 in citrate-phosphate buffer solution, pH 4.5, are used. The sensitivity of the method is 5 X 10(4) microbial bodies per ml.
摘要
利用磁性聚丙烯酰胺微粒在磁性酶免疫测定中检测鼠疫耶尔森氏菌细胞。在用于测定抗原的分析系统中，使用市售的鼠疫耶尔森氏菌抗原、过氧化物酶标记抗体以及由2,2-偶氮二（3-乙基苯并噻唑啉-6-磺酸）钠盐和过氧化氢在pH 4.5的柠檬酸-磷酸盐缓冲溶液中组成的底物混合物。该方法的灵敏度为每毫升5×10⁴个微生物体。
Zotero 插件