[使用纸盘的免疫酶分析]
[Immunoenzyme analysis using paper disks].
作者信息
Levi M I, Iagovkina V N
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1988 Dec(12):94-9.
PMID:3072810
Abstract
Filter paper discs have been used in the enzyme immunoassay (EIA) as solid phase instead of polystyrene plates. The use of paper discs has made it possible to achieve a multiple increase in the sensitivity of sandwich EIA, thus permitting the detection of Yersinia pestis capsular antigen at a concentration of 0.4 ng/ml. Paper discs can be used not only for the sorption of antigen and antibodies, but also for the affinity purification of preparations.
摘要
滤纸圆盘已在酶免疫测定（EIA）中用作固相，取代了聚苯乙烯板。使用滤纸圆盘使得夹心酶免疫测定的灵敏度能够提高数倍，从而能够检测浓度为0.4 ng/ml的鼠疫耶尔森菌荚膜抗原。滤纸圆盘不仅可用于吸附抗原和抗体，还可用于制剂的亲和纯化。
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