School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea.
School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea.
Bioresour Technol. 2016 Apr;205:205-12. doi: 10.1016/j.biortech.2016.01.054. Epub 2016 Jan 23.
Direct interspecies electron transfer (DIET) via conductive materials can provide significant benefits to anaerobic methane formation in terms of production amount and rate. Although granular activated carbon (GAC) demonstrated its applicability in facilitating DIET in methanogenesis, DIET in continuous flow anaerobic reactors has not been verified. Here, evidences of DIET via GAC were explored. The reactor supplemented with GAC showed 1.8-fold higher methane production rate than that without GAC (35.7 versus 20.1±7.1mL-CH4/d). Around 34% of methane formation was attributed to the biomass attached to GAC. Pyrosequencing of 16S rRNA gene demonstrated the enrichment of exoelectrogens (e.g. Geobacter) and hydrogenotrophic methanogens (e.g. Methanospirillum and Methanolinea) from the biomass attached to GAC. Furthermore, anodic and cathodic currents generation was observed in an electrochemical cell containing GAC biomass. Taken together, GAC supplementation created an environment for enriching the microorganisms involved in DIET, which increased the methane production rate.
通过导电材料进行直接种间电子转移 (DIET) 可以在产气量和产率方面为厌氧甲烷形成提供显著的益处。虽然颗粒活性炭 (GAC) 已被证明在促进产甲烷作用中的 DIET 方面具有适用性，但在连续流动厌氧反应器中的 DIET 尚未得到验证。在这里，探索了通过 GAC 进行 DIET 的证据。补充 GAC 的反应器的甲烷产生速率比没有 GAC 的反应器高 1.8 倍（35.7 与 20.1±7.1mL-CH4/d）。约 34%的甲烷形成归因于附着在 GAC 上的生物量。16S rRNA 基因的焦磷酸测序表明，从附着在 GAC 上的生物量中富集了异化电子供体（例如 Geobacter）和氢营养型产甲烷菌（例如 Methanospirillum 和 Methanolinea）。此外，在含有 GAC 生物量的电化学电池中观察到阳极和阴极电流的产生。综上所述，GAC 的补充为富集参与 DIET 的微生物创造了环境，从而提高了甲烷的产生速率。
