Valentin Marvin T, Świechowski Kacper, Białowiec Andrzej
Department of Applied Bioeconomy, Wrocław University of Environmental and Life Sciences, 51-630 Wroclaw, Poland.
Benguet State University, Km. 5, La Trinidad, Benguet 2601, Philippines.
Materials (Basel). 2023 Oct 11;16(20):6655. doi: 10.3390/ma16206655.
The application of biochar as an additive to enhance the anaerobic digestion (AD) of biomass has been extensively studied from various perspectives. This study reported, for the first time, the influence of biochar incubation in the inoculum on the anaerobic fermentation of glucose in a batch-type reactor over 20 days. Three groups of inoculum with the same characteristics were pre-mixed once with biochar for different durations: 21 days (D21), 10 days (D10), and 0 days (D0). The BC was mixed in the inoculum at a concentration of 8.0 g/L. The proportion of the inoculum and substrate was adjusted to an inoculum-to-substrate ratio of 2.0 based on the volatile solids. The results of the experiment revealed that D21 had the highest cumulative methane yield, of 348.98 mL, compared to 322.66, 290.05, and 25.15 mL obtained from D10, D0, and the control, respectively. Three models-modified Gompertz, first-order, and Autoregressive Integrated Moving Average (ARIMA)-were used to interpret the biomethane production. All models showed promising fitting of the cumulative biomethane production, as indicated by high R2 and low RMSE values. Among these models, the ARIMA model exhibited the closest fit to the actual data. The biomethane production rate, derived from the modified Gompertz Model, increased as the incubation period increased, with D21 yielding the highest rate of 31.13 mL/gVS. This study suggests that the application of biochar in the anaerobic fermentation of glucose, particularly considering the short incubation period, holds significant potential for improving the overall performance of anaerobic digestion.
作为一种添加剂，生物炭在增强生物质厌氧消化（AD）方面的应用已从多个角度进行了广泛研究。本研究首次报道了在批式反应器中，接种物中生物炭的预培养对葡萄糖厌氧发酵20天的影响。将三组具有相同特性的接种物分别与生物炭预混合不同时长：21天（D21）、10天（D10）和0天（D0）。生物炭以8.0 g/L的浓度混入接种物中。根据挥发性固体将接种物与底物的比例调整为接种物与底物之比为2.0。实验结果表明，D21的累积甲烷产量最高，为348.98 mL，而D10、D0和对照组分别为322.66 mL、290.05 mL和25.15 mL。使用了三种模型——修正的Gompertz模型、一阶模型和自回归积分移动平均（ARIMA）模型来解释生物甲烷的产生。所有模型对累积生物甲烷产量的拟合效果都很有前景，R2值高且RMSE值低表明了这一点。在这些模型中，ARIMA模型与实际数据的拟合度最高。根据修正的Gompertz模型得出的生物甲烷产率随着培养时间的增加而增加，D21的产率最高，为31.13 mL/gVS。本研究表明，生物炭在葡萄糖厌氧发酵中的应用，特别是考虑到较短的培养期，在提高厌氧消化的整体性能方面具有巨大潜力。
