Feliciano Rodrigo P, Boeres Albert, Massacessi Luca, Istas Geoffrey, Ventura M Rita, Nunes Dos Santos Cláudia, Heiss Christian, Rodriguez-Mateos Ana
Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, University of Duesseldorf, D-40225 Dusseldorf, Germany.
Division of Cardiology, Pulmonology, and Vascular Medicine, Medical Faculty, University of Duesseldorf, D-40225 Dusseldorf, Germany; QUVI Department for Life Quality Studies, University of Bologna, Italy.
Arch Biochem Biophys. 2016 Jun 1;599:31-41. doi: 10.1016/j.abb.2016.01.014. Epub 2016 Feb 4.
Cranberries are a rich source of (poly)phenols, in particular proanthocyanidins, anthocyanins, flavonols, and phenolic acids. However, little is known about their bioavailability in humans. We investigated the absorption, metabolism, and excretion of cranberry (poly)phenols in plasma and urine of healthy young men after consumption of a cranberry juice (787 mg (poly)phenols). A total of 60 cranberry-derived phenolic metabolites were identified using UPLC-Q-TOF-MS analysis with authentic standards. These included sulfates of pyrogallol, valerolactone, benzoic acids, phenylacetic acids, glucuronides of flavonols, as well as sulfates and glucuronides of cinnamic acids. The most abundant plasma metabolites were small phenolic compounds, in particular hippuric acid, catechol-O-sulfate, 2,3-dihydroxybenzoic acid, phenylacetic acid, isoferulic acid, 4-methylcatechol-O-sulfate, α-hydroxyhippuric acid, ferulic acid 4-O-sulfate, benzoic acid, 4-hydroxyphenyl acetic acid, dihydrocaffeic acid 3-O-sulfate, and vanillic acid-4-O-sulfate. Some benzoic acids, cinnamic acids, and flavonol metabolites appeared in plasma early, at 1-2 h post-consumption. Others such as phenylacetic acids, benzaldehydes, pyrogallols, catechols, hippuric and dihydrocinnamic acid derivatives appear in plasma later (Tmax 4-22 h). The 24 h urinary recovery with respect to the amount of (poly)phenols consumed was 6.2%. Our extensive description of the bioavailability of cranberry (poly)phenols lays important groundwork necessary to start understanding the fate of these compounds in humans.
蔓越莓富含（多）酚类物质，尤其是原花青素、花青素、黄酮醇和酚酸。然而，人们对它们在人体内的生物利用度知之甚少。我们研究了健康年轻男性饮用蔓越莓汁（含787毫克（多）酚）后，蔓越莓（多）酚在血浆和尿液中的吸收、代谢及排泄情况。通过超高效液相色谱-四极杆飞行时间质谱联用仪（UPLC-Q-TOF-MS）分析及使用标准品，共鉴定出60种源自蔓越莓的酚类代谢物。这些代谢物包括焦性没食子酸硫酸盐、戊内酯、苯甲酸、苯乙酸、黄酮醇葡萄糖醛酸苷，以及肉桂酸硫酸盐和葡萄糖醛酸苷。血浆中含量最丰富的代谢物是小分子酚类化合物，尤其是马尿酸、儿茶酚-O-硫酸盐、2,3-二羟基苯甲酸、苯乙酸、异阿魏酸、4-甲基儿茶酚-O-硫酸盐、α-羟基马尿酸、阿魏酸4-O-硫酸盐、苯甲酸、4-羟基苯乙酸、二氢咖啡酸3-O-硫酸盐和香草酸-4-O-硫酸盐。一些苯甲酸、肉桂酸和黄酮醇代谢物在饮用后1至2小时较早出现在血浆中。其他代谢物如苯乙酸、苯甲醛、焦性没食子酸、儿茶酚、马尿酸和二氢肉桂酸衍生物则在较晚时间（达峰时间为4至22小时）出现在血浆中。相对于摄入的（多）酚量，24小时尿液回收率为6.2%。我们对蔓越莓（多）酚生物利用度的详细描述为开始了解这些化合物在人体内的去向奠定了重要基础。
