Zhang Kai, Zuo Yuegang
Department of Chemistry and Biochemistry, University of Massachusetts Dartmouth, 285 Old Westport Road, North Dartmouth, Massachusetts 02747, USA.
J Agric Food Chem. 2004 Jan 28;52(2):222-7. doi: 10.1021/jf035073r.
A GC-MS method was developed for the determination of various flavonoids and phenolic and benzoic acids in human plasma. The procedure involved the extraction of flavonoids and phenolic and benzoic acids with ethyl acetate, followed by the derivatization of the phenolic and benzoic compounds with BSTFA (N,O-bis(trimethylsilyl) trifluoroacetamide) + TMCS (trimethylchlorosilane) reagent. The trimethylsilyl derivatives formed were separated and quantitated using GC-MS. Twenty flavonoids and phenolic and benzoic compounds have been well separated in the spiked human plasma without any interference. The average recovery was 79.3%. Several phenolic acids such as o-hydroxybenzoic, p-hydroxyphenylacetic, 2,3-dihydroxybenzoic, 2,4-dihydroxybenzoic, ferulic, sinapic, and benzoic acid were identified and quantified in human plasma after consumption of a cranberry juice. This developed method provides a simple, specific, and sensitive technique for the simultaneous determination of flavonoids and phenolic and benzoic acids in human plasma and is suitable for bioavailability and pharmacokinetic studies.
建立了一种气相色谱 - 质谱（GC-MS）法，用于测定人血浆中的各种黄酮类化合物、酚酸和苯甲酸。该方法包括用乙酸乙酯萃取黄酮类化合物、酚酸和苯甲酸，然后用BSTFA（N，O-双（三甲基硅基）三氟乙酰胺）+ TMCS（三甲基氯硅烷）试剂对酚类和苯甲酸化合物进行衍生化。使用GC-MS对形成的三甲基硅基衍生物进行分离和定量。在加标的人血浆中，20种黄酮类化合物、酚类和苯甲酸化合物已得到很好的分离，没有任何干扰。平均回收率为79.3%。在饮用蔓越莓汁后，在人血浆中鉴定并定量了几种酚酸，如邻羟基苯甲酸、对羟基苯乙酸、2,3 - 二羟基苯甲酸、2,4 - 二羟基苯甲酸、阿魏酸、芥子酸和苯甲酸。这种开发的方法为同时测定人血浆中的黄酮类化合物、酚酸和苯甲酸提供了一种简单、特异且灵敏的技术，适用于生物利用度和药代动力学研究。
