State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China.
Bioresour Technol. 2016 Apr;206:9-15. doi: 10.1016/j.biortech.2016.01.066. Epub 2016 Jan 25.
An efficient aerobic denitrification bacterium, strain NNA5, was isolated and identified as Marinobacter sp. NNA5. NNA5 did not perform heterotrophic nitrification. GC/IRMS analysis revealed that (15)N2 was produced from Na(15)NO2 and K(15)NO3. GC/MS and quantitative analyses showed that no N2O emission occurred when nitrite or nitrate was used as substrate. Single factor experiments indicated that optimal conditions for aerobic denitrification were: sodium succinate or sodium pyruvate as carbon source, temperature 35 °C, NaCl concentration 2-4%, C/N ratio 6-8, pH 7.5, rotation speed 150 rpm (giving dissolved oxygen concentration 6.08 mg/L), NO3(-)-N concentration ranging from 140 to 700 mg/L. NNA5 displayed highly efficient aerobic denitrifying ability, with maximal NO3(-)-N removal rate 112.8 mg/L/d. In view of its ability to perform aerobic denitrification with zero N2O emission, NNA5 has great potential for future application in aerobic denitrification processes in industrial and aquaculture wastewater treatment systems.
一株高效好氧反硝化菌 NNA5 被分离并鉴定为 Marinobacter sp. NNA5。NNA5 不进行异养硝化。GC/IRMS 分析表明，(15)N2 是由 Na(15)NO2 和 K(15)NO3 产生的。GC/MS 和定量分析表明，当使用亚硝酸盐或硝酸盐作为底物时，不会发生 N2O 排放。单因素实验表明，好氧反硝化的最佳条件为：琥珀酸钠或丙酮酸作为碳源，温度 35°C，NaCl 浓度 2-4%，C/N 比 6-8，pH7.5，转速 150rpm（溶氧浓度 6.08mg/L），NO3(-)-N 浓度为 140-700mg/L。NNA5 表现出高效的好氧反硝化能力，最大 NO3(-)-N 去除率为 112.8mg/L/d。鉴于其在零 N2O 排放条件下进行好氧反硝化的能力，NNA5 在未来工业和水产养殖废水处理系统中的好氧反硝化过程中有很大的应用潜力。
