Key Laboratory of Coal Science and Technology, Ministry of Education and Shanxi Province, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, China.
Innovation Institute of Environmental Industry, College of Environmental Science and Engineering, Taiyuan University of Technology, Taiyuan, 030024, Shanxi, China.
Bioprocess Biosyst Eng. 2021 Feb;44(2):391-401. doi: 10.1007/s00449-020-02451-0. Epub 2020 Sep 27.
A new aerobic denitrifying bacterium ZYL was isolated from domestic wastewater sludge and identified as Acinetobacter haemolyticus (similarity 99%) by the 16S rDNA sequencing analysis. The strain could use nitrate, nitrite and ammonium as the sole N-source for growth with a final product of N, demonstrating its good abilities for aerobic denitrification and heterotrophic nitrification. Single-factor experiment results showed that the effective removal of nitrate by strain ZYL occurred with carbon source sodium succinate, C/N 16-24, pH 5-9, temperature 20-40 °C, DO ≥ 4.84 mg/L. Ammonium was preferentially used by strain ZYL with nitrate and ammonium as the mixed nitrogen sources. According to nitrogen utilization, nitrogen balance analysis, enzyme assay and denitrifying gene amplification, nitrate was assimilated directly by the isolate for cell synthesis and also converted into N through aerobic denitrification. All these make strain ZYL an ideal choice for treating nitrogen-containing wastewater.
从生活污水污泥中分离得到一株好氧反硝化菌 ZYL，经 16S rDNA 测序分析鉴定为溶血不动杆菌（相似度为 99%）。该菌株可以利用硝酸盐、亚硝酸盐和铵盐作为唯一氮源进行生长，最终产物为氮气，表明其具有良好的好氧反硝化和异养硝化能力。单因素实验结果表明，菌株 ZYL 有效去除硝酸盐的条件为：碳源琥珀酸钠，C/N 16-24，pH 5-9，温度 20-40°C，DO≥4.84mg/L。当硝酸盐和铵盐作为混合氮源时，菌株 ZYL 优先利用铵盐。根据氮素利用、氮素平衡分析、酶活性测定和反硝化基因扩增，该菌可直接将硝酸盐同化用于细胞合成，也可通过好氧反硝化转化为氮气。所有这些使菌株 ZYL 成为处理含氮废水的理想选择。
