[从临床样本中分离的热带念珠菌毒力因子研究]
[Study on virulence factors of Candida tropicalis isolated from clinical samples].
作者信息
Yu Shuanbao, Li Wenge, Che Jie, Bian Funing, Lu Jinxing, Wu Yuan
机构信息
State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
State Key Laboratory for Communicable Diseases Prevention and Control, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China; Email:
出版信息
Zhonghua Liu Xing Bing Xue Za Zhi. 2015 Oct;36(10):1162-6.
OBJECTIVE
To determine the in vitro production of virulence factors for Candida (C.) tropicalis, including aspartyl proteinases, phospholipases and hemolytic activities, describe the regulation of virulence factors varying with time in C. tropicalis, and analyze the differences in aspartyl proteinases and hemolytic activities of C. tropicalis isolated from anatomically distinct sites.
METHODS
A total of 64 C. tropicalis strains were spot-inoculated onto bovine albumin agar, egg yolk agar and sheep blood agar plates, respectively. Then the plates were incubated for 24, 48 and 72 hour at 37 °C, respectively. The aspartyl proteinases, phospholipase and hemolytic activities were determined at each time point, respectively.
RESULTS
All the C. tropiclais isolates showed positive aspartyl proteinases and hemolytic activities at each time point, but no phospholipases activity was detected in C. tropicalis. On comparison of aspartyl proteinases and hemolytic activities at different time points, aspartyl proteinases activity at 48 and 72 hour was higher than that at 24 hour. During 72 hour, hemolytic activity of C. tropicalis increased. No statistical significant differences in aspartyl proteinases and hemolytic activities of C. tropicalis were observed among different infection sites (P=0.368 and 0.985).
CONCLUSION
The C. tropicalis clinical isolates in China have aspartyl proteinases activity, hemolytic activity, but have no phospholipase activity.
目的
测定热带假丝酵母菌毒力因子的体外产生情况，包括天冬氨酸蛋白酶、磷脂酶和溶血活性，描述热带假丝酵母菌毒力因子随时间的变化规律，并分析从不同解剖部位分离的热带假丝酵母菌在天冬氨酸蛋白酶和溶血活性方面的差异。
方法
将总共64株热带假丝酵母菌分别点种在牛血清白蛋白琼脂、蛋黄琼脂和羊血琼脂平板上。然后将平板分别在37℃孵育24、48和72小时。在每个时间点分别测定天冬氨酸蛋白酶、磷脂酶和溶血活性。
结果
所有热带假丝酵母菌分离株在每个时间点均显示出天冬氨酸蛋白酶和溶血活性阳性，但未检测到热带假丝酵母菌的磷脂酶活性。比较不同时间点的天冬氨酸蛋白酶和溶血活性，48小时和72小时的天冬氨酸蛋白酶活性高于24小时。在72小时内，热带假丝酵母菌的溶血活性增加。在不同感染部位，热带假丝酵母菌的天冬氨酸蛋白酶和溶血活性未观察到统计学显著差异（P = 0.368和0.985）。
结论
中国的热带假丝酵母菌临床分离株具有天冬氨酸蛋白酶活性、溶血活性，但无磷脂酶活性。
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